Any feedback?
Literature summary extracted from
- Expression and characterization of protein disulfide isomerase family proteins in bread wheat (2015), BMC Plant Biol., 15, 73 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.4.1 | expressed in Escherichia coli BL21(DE3) cells | Triticum aestivum |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.4.1 | endoplasmic reticulum | - |
Triticum aestivum | 5783 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.4.1 | reduced ribonuclease A | Triticum aestivum | - |
denatured ribonuclease A | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.4.1 | Triticum aestivum | A0A024FRA5 | - |
- |
| 5.3.4.1 | Triticum aestivum | A0A024FRN3 | - |
- |
| 5.3.4.1 | Triticum aestivum | A0A024FRN8 | - |
- |
| 5.3.4.1 | Triticum aestivum | D8L9A3 | - |
- |
| 5.3.4.1 | Triticum aestivum | D8L9B3 | - |
- |
| 5.3.4.1 | Triticum aestivum | Q7FYS2 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.4.1 | Ni-Sepharose 6 column chromatography, and TSK gel G3000SW gel filtration | Triticum aestivum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 5.3.4.1 | aleurone layer | - |
Triticum aestivum | - |
| 5.3.4.1 | endosperm | - |
Triticum aestivum | - |
| 5.3.4.1 | seed | - |
Triticum aestivum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.4.1 | 0.175 | - |
isoform PDIL5A, at pH 7.5 and 25°C | Triticum aestivum |
| 5.3.4.1 | 0.179 | - |
isoform PDIL4D, at pH 7.5 and 25°C | Triticum aestivum |
| 5.3.4.1 | 0.287 | - |
isoform PDIL2, at pH 7.5 and 25°C | Triticum aestivum |
| 5.3.4.1 | 0.665 | - |
isoform PDIL1B, at pH 7.5 and 25°C | Triticum aestivum |
| 5.3.4.1 | 0.736 | - |
isoform PDIL1Aalpha, at pH 7.5 and 25°C | Triticum aestivum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.4.1 | reduced ribonuclease A | - |
Triticum aestivum | denatured ribonuclease A | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | ? | x * 40000, isoform PDIL4D, SDS-PAGE | Triticum aestivum |
| 5.3.4.1 | ? | x * 47000, isoform PDIL5A, SDS-PAGE | Triticum aestivum |
| 5.3.4.1 | ? | x * 60000, isoform PDIL1Aalpha, SDS-PAGE | Triticum aestivum |
| 5.3.4.1 | ? | x * 60000, isoform PDIL1B, SDS-PAGE | Triticum aestivum |
| 5.3.4.1 | ? | x * 69000, isoform PDIL3A, SDS-PAGE | Triticum aestivum |
| 5.3.4.1 | ? | x * 70000, isoform PDIL2, SDS-PAGE | Triticum aestivum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.4.1 | PDIL1Aalpha | isoform | Triticum aestivum |
| 5.3.4.1 | PDIL1B | isoform | Triticum aestivum |
| 5.3.4.1 | PDIL2 | isoform | Triticum aestivum |
| 5.3.4.1 | PDIL3A | isoform | Triticum aestivum |
| 5.3.4.1 | PDIL4D | isoform | Triticum aestivum |
| 5.3.4.1 | PDIL5A | isoform | Triticum aestivum |
| 5.3.4.1 | protein disulfide isomerase | - |
Triticum aestivum |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
