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Literature summary extracted from

  • Yildirim, D.; Tükel, S.S.; Alagöz, D.
    Crosslinked enzyme aggregates of hydroxynitrile lyase partially purified from Prunus dulcis seeds and its application for the synthesis of enantiopure cyanohydrins (2014), Biotechnol. Prog., 30, 818-827 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.2.10 synthesis the immobilization of partially purified hydroxynitrile lyase from Prunus dulcis as crosslinked enzyme aggregate is a very promising tool owing to its ease of preparation, cheapness, and efficiency in the preparation of enantiopure cyanohydrins. Response surface methodology provides an effective way for the optimization of the preparation of PdHNL-crosslinked enzyme aggregate. The synthesis of (R)-mandelonitrile, (R)-2-chloromandelonitrile, (R)-3,4-dihydroxymandelonitrile, (R)-2-hydroxy-4-phenyl butyronitrile, (R)-4-bromomandelonitrile, (R)-4-fluoromandelonitrile, and (R)-4-nitromandelonitrile is achieved with high yield and enantiomeric excess Prunus dulcis

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.10 Prunus dulcis
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-
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Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.10 partial Prunus dulcis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.10 seed
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 Prunus dulcis
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.10 cyanide + 2-chlorobenzaldehyde synthesis of (R)-2-chloromandelonitrile with a yield of 100% and an enantiomeric excess of 21% Prunus dulcis (R)-2-chloromandelonitrile
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 ?
4.1.2.10 cyanide + 3,4-dihydroxybenzaldehyde synthesis of (R)-3,4-dihydroxymandelonitrile with a yield of 100% and an enantiomeric excess of 99% Prunus dulcis (R)-3,4-dihydroxymandelonitrile
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 ?
4.1.2.10 cyanide + 3-phenylpropionaldehyde synthesis of (R)-2-hydroxy-4-phenyl butyronitrile with a yield of 83% and an enantiomeric excess of 91% Prunus dulcis (R)-2-hydroxy-4-phenyl butyronitrile
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 ?
4.1.2.10 cyanide + 4-bromobenzaldehyde synthesis of (R)-4-bromomandelonitrile with a yield pf 100% and an enantiomeric excess value of 99% Prunus dulcis (R)-4-bromomandelonitrile
-
 ?
4.1.2.10 cyanide + 4-fluorobenzaldehyde synthesis of (R)-4-fluoromandelonitrile with a yield of 100% and an enantiomeric excess value of 72% Prunus dulcis (R)-4-fluoromandelonitrile
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 ?
4.1.2.10 cyanide + 4-nitrobenzaldehyde synthesis of (R)-4-nitromandelonitrile with a yield of 100% and an enantiomeric excess value of 14% Prunus dulcis (R)-4-nitromandelonitrile
-
 ?
4.1.2.10 cyanide + benzaldehyde synthesis of (R)-mandelonitrile with a yield of 100% and an enantiomeric excess of 99% Prunus dulcis (R)-mandelonitrile
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 ?