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Literature summary extracted from

  • Williams, A.; He, W.; Cress, B.F.; Liu, X.; Alexandria, J.; Yoshizawa, H.; Nishimura, K.; Toida, T.; Koffas, M.; Linhardt, R.J.
    Cloning and expression of recombinant chondroitinase ACII and its comparison to the Arthrobacter aurescens enzyme (2017), Biotechnol. J., 12, 1700239 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.2.2.5 synthesis the enzyme can serve as a suitable replacement for the original enzyme, from Arthrobacter aurescens IAM 110 65, UniProt ID P84141, which is no longer commercially available Paenarthrobacter aurescens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.5 expression in Escherichia coli Paenarthrobacter aurescens

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.5 I236T mutation introduced to render the enzyme identical to ACII from Arthrobacter aurescens IAM 110 65. Mutant behaves similar to wild-type Paenarthrobacter aurescens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.5 extracellular
-
 Paenarthrobacter aurescens
-
-

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Paenarthrobacter aurescens
-
-
-
4.2.2.5 Paenarthrobacter aurescens 161MFSha2.1
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.2.5 culture fluid
-
 Paenarthrobacter aurescens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.5 4
-
 pH 7.4, 37°C Paenarthrobacter aurescens

Storage Stability

EC Number Storage Stability Organism
4.2.2.5 -40°C, no loss by freeze-drying in presence of 100 mM lactose or trehalose, about 60% of enzymatic activity is lost on long-term storage in the absence of both trehalose and lactose Paenarthrobacter aurescens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.5 chondroitin sulfate A
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 chondroitin sulfate A
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
4.2.2.5 chondroitin sulfate B
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 chondroitin sulfate B
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
4.2.2.5 chondroitin sulfate C
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 chondroitin sulfate C
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
4.2.2.5 chondroitin sulfate D
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 chondroitin sulfate D
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?
4.2.2.5 chondroitin sulfate E
-
 Paenarthrobacter aurescens ?
-
 ?
4.2.2.5 chondroitin sulfate E
-
 Paenarthrobacter aurescens 161MFSha2.1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.5 ? x * 83300, calculated from sequence, x * 75000, SDS-PAGE Paenarthrobacter aurescens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.5 37
-
-
 Paenarthrobacter aurescens

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.2.5 50
-
 10% residual activity Paenarthrobacter aurescens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.5 Paenarthrobacter aurescens calculated from sequence
-
 12