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Literature summary extracted from

  • Vo, T.; Pham, V.; Ko, J.; Lee, S.; Park, S.; Hong, S.
    Improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli (2014), Biotechnol. Bioprocess Eng., 19, 327-331 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.15 gene gadB, recombinant expression in GABA aminotransferase-knockout Escherichia coli strain XBT from plasmid pHBP, subcloning in Escherichia coli strain XL-1 Blue Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.15 additional information improvement of gamma-amino butyric acid production by an overexpression of glutamate decarboxylase from Pyrococcus horikoshii in Escherichia coli, optimization of GABA production method, overview. The highest final GABA concentration, 5.07 g/l, is obtained from 10 g/l of monosodium glutamate with a GABA yield of 83% at 30ºC and pH 3.5. When Pyrococcus horikoshii glutamate decarboxylase is introduced into a GABA aminotransferase knockout Escherichia coli XBT strain, 5.69 g/l of GABA is produced with a GABA yield of 93% Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.15 L-glutamate Pyrococcus horikoshii
-
 4-aminobutanoate + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.15 Pyrococcus horikoshii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.15 L-glutamate
-
 Pyrococcus horikoshii 4-aminobutanoate + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.15 GAD
-
 Pyrococcus horikoshii
4.1.1.15 GadB
-
 Pyrococcus horikoshii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.15 30
-
 recombinant enzyme in Escherichia coli Pyrococcus horikoshii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.15 3.5
-
 recombinant enzyme in Escherichia coli Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.15 pyridoxal 5'-phosphate
-
 Pyrococcus horikoshii