Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Iinoya, K.; Kotani, T.; Sasano, Y.; Takagi, H.
    Engineering of the yeast antioxidant enzyme Mpr1 for enhanced activity and stability (2009), Biotechnol. Bioeng., 103, 341-352 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.271 expressed in Saccharomyces cerevisiae strains S288C and CKY8 Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.271 F65G the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 F65L the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 F65L/L117V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 F65L/L117V the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type Saccharomyces cerevisiae
2.3.1.271 K63P the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 K63R the mutant shows higher azetidine-2-carboxylate resistance compared to the wild type Saccharomyces cerevisiae
2.3.1.271 K63R/F65L the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 K63R/F65L/L117V the mutant shows reduced catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 L117V the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae
2.3.1.271 LK63R/L117V the mutant shows higher catalytic efficiency compared to the wild type enzyme Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.271 0.0049
-
 acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0076
-
 acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.008
-
 acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.01
-
 acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.011
-
 acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.013
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.013
-
 acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.019
-
 acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.019
-
 acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.14
-
 acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.7
-
 azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.2
-
 azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.2
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.4
-
 azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.6
-
 azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.6
-
 azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.7
-
 azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 1.8
-
 azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 2.2
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 3.3
-
 azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.271 acetyl-CoA + L-glutamate-5-semialdehyde Saccharomyces cerevisiae
-
 CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?
2.3.1.271 acetyl-CoA + L-glutamate-5-semialdehyde Saccharomyces cerevisiae Sigma1278b
-
 CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.271 Saccharomyces cerevisiae E9P8D2
-
-
2.3.1.271 Saccharomyces cerevisiae Sigma1278b E9P8D2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.271 Ni-NTA column chromatography Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.271 acetyl-CoA + azetidine-2-carboxylate
-
 Saccharomyces cerevisiae CoA + N-acetyl-azetidine-2-carboxylate
-
 ?
2.3.1.271 acetyl-CoA + azetidine-2-carboxylate
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-azetidine-2-carboxylate
-
 ?
2.3.1.271 acetyl-CoA + L-glutamate-5-semialdehyde
-
 Saccharomyces cerevisiae CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?
2.3.1.271 acetyl-CoA + L-glutamate-5-semialdehyde
-
 Saccharomyces cerevisiae Sigma1278b CoA + N-acetyl-L-glutamate 5-semialdehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.271 MPR1
-
 Saccharomyces cerevisiae

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.271 50
-
 the enzyme has a half-life of 8.2 min at 50°C Saccharomyces cerevisiae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.271 3 6 azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 9.1
-
 acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 11
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 15
-
 acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 16
-
 azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 17
-
 acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 19
-
 azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 27
-
 acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 31
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 31
-
 acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 31
-
 azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 31
-
 acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 31
-
 azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 38
-
 azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 38
-
 acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 38
-
 acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 42
-
 acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 46
-
 azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 48
-
 azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 51
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.1.271 0.00012
-
 acetyl-CoA mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0008
-
 acetyl-CoA mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0022
-
 acetyl-CoA mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0023
-
 acetyl-CoA mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0023
-
 acetyl-CoA mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0025
-
 acetyl-CoA wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0029
-
 acetyl-CoA mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0033
-
 acetyl-CoA mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.0037
-
 acetyl-CoA mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 0.005
-
 acetyl-CoA mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 4.7
-
 azetidine-2-carboxylate mutant enzyme F65G, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 9.5
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 12
-
 azetidine-2-carboxylate mutant enzyme K63P, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 21
-
 azetidine-2-carboxylate mutant enzyme F65L/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 22
-
 azetidine-2-carboxylate wild type enzyme, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 23
-
 azetidine-2-carboxylate mutant enzyme K63R/F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 25
-
 azetidine-2-carboxylate mutant enzyme K63R, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 27
-
 azetidine-2-carboxylate mutant enzyme LK63R/L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 33
-
 azetidine-2-carboxylate mutant enzyme L117V, at pH 8.5 and 30°C Saccharomyces cerevisiae
2.3.1.271 46
-
 azetidine-2-carboxylate mutant enzyme F65L, at pH 8.5 and 30°C Saccharomyces cerevisiae