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Literature summary extracted from

  • Noda, S.; Kawai, Y.; Tanaka, T.; Kondo, A.
    4-Vinylphenol biosynthesis from cellulose as the sole carbon source using phenolic acid decarboxylase- and tyrosine ammonia lyase-expressing Streptomyces lividans (2015), Biores. Technol., 180, 59-65 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.1.1.102 synthesis for 4-vinylphenol production directly from cellulose, L-tyrosine ammonia lyase derived from Rhodobacter sphaeroides and phenolic acid decarboxylase from Streptomyces sviceus are introduced into endoglucanase-secreting Streptomyces lividans, and the 4-vinylphenol biosynthetic pathway is constructed therein. The created transformants successfully produce 4-vinylphenol directly from cellulose Streptomyces sviceus
4.3.1.23 synthesis for 4-vinylphenol production directly from cellulose, L-tyrosine ammonia lyase derived from Rhodobacter sphaeroides and phenolic acid decarboxylase from Streptomyces sviceus are introduced into endoglucanase-secreting Streptomyces lividans, and the 4-vinylphenol biosynthetic pathway is constructed therein. The created transformants successfully produce 4-vinylphenol directly from cellulose Cereibacter sphaeroides

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.1.102 expression in Escherichia coli BL21 (DE3) Streptomyces hygroscopicus
4.1.1.102 expression in Escherichia coli BL21 (DE3) Streptomyces sviceus
4.1.1.102 expression in Escherichia coli BL21 (DE3) Streptomyces cattleya
4.1.1.102 expression in Streptomyces lividans Streptomyces sviceus
4.3.1.23 expression in Streptomyces lividans Cereibacter sphaeroides

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.102 0.54
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces cattleya
4.1.1.102 0.74
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces sviceus
4.1.1.102 1.07
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces hygroscopicus

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.102 Streptomyces cattleya F8JS69
-
-
4.1.1.102 Streptomyces hygroscopicus
-
-
-
4.1.1.102 Streptomyces sviceus B5HL29
-
-
4.3.1.23 Cereibacter sphaeroides Q3IWB0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.102
-
 Streptomyces hygroscopicus
4.1.1.102
-
 Streptomyces sviceus
4.1.1.102
-
 Streptomyces cattleya

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.102 4-hydroxycinnamic acid
-
 Streptomyces hygroscopicus 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-hydroxycinnamic acid
-
 Streptomyces sviceus 4-vinylphenol + CO2
-
 ?
4.1.1.102 4-hydroxycinnamic acid
-
 Streptomyces cattleya 4-vinylphenol + CO2
-
 ?
4.3.1.23 L-tyrosine
-
 Cereibacter sphaeroides trans-p-hydroxycinnamate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.1.102 PAD
-
 Streptomyces hygroscopicus
4.1.1.102 PAD
-
 Streptomyces sviceus
4.1.1.102 PAD
-
 Streptomyces cattleya
4.1.1.102 phenolic acid decarboxylase
-
 Streptomyces hygroscopicus
4.1.1.102 phenolic acid decarboxylase
-
 Streptomyces sviceus
4.1.1.102 phenolic acid decarboxylase
-
 Streptomyces cattleya

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.1.102 30
-
-
 Streptomyces hygroscopicus
4.1.1.102 30
-
-
 Streptomyces sviceus
4.1.1.102 30
-
-
 Streptomyces cattleya
4.3.1.23 30
-
-
 Cereibacter sphaeroides

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.1.1.102 10 50 10°C: about 70% of maximal activity, 50°C: about 40% of maximal activity Streptomyces sviceus
4.1.1.102 10 30 10°C: about 39% of maximal activity, 30°C: maximal activity, 40°C: no activity Streptomyces hygroscopicus
4.1.1.102 20 50 20°C: about 70% of maximal activity, 50°C: about 30% of maximal activity Streptomyces cattleya

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.102 0.19
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces cattleya
4.1.1.102 0.96
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces sviceus
4.1.1.102 14.1
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces hygroscopicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.102 4
-
-
 Streptomyces sviceus
4.1.1.102 5 6
-
 Streptomyces hygroscopicus
4.1.1.102 5 6
-
 Streptomyces cattleya

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.102 4 7 pH 4.0: about 30% of maximal activity, pH 7.0: about 60% of maximal activity Streptomyces cattleya
4.1.1.102 4 7 pH 4.0: maximal activity, pH 7.0: about 65% of maximal activity Streptomyces sviceus
4.1.1.102 5 7 pH 5.0-6.0: maximal activity, pH 7.0: about 45% of maximal activity Streptomyces hygroscopicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.1.102 4
-
 inactivated at Streptomyces hygroscopicus
4.1.1.102 4
-
 inactivated at Streptomyces cattleya

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.1.102 0.013
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces hygroscopicus
4.1.1.102 0.35
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces cattleya
4.1.1.102 1.31
-
 4-hydroxycinnamic acid pH 5, 30°C Streptomyces sviceus