EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.99.2 | synthesis | the tyrosine phenol lyase (TPL) catalyzed synthesis of L-DOPA is regarded as one of the most economic routes for L-DOPA synthesis | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.2 | gene tnaA, recombinant expression in Escherichia coli strain BL21(DE3) | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.99.2 | additional information | the tyrosine phenol lyase (TPL) catalyzed synthesis of L-DOPA is regarded as one of the most economic routes for L-DOPA synthesis, usage in a fed-batch approach with sodium pyruvate, dicatechol and ammonium acetate. In addition, L-DOPA crystals are exogenously added to inhibit cell encapsulation by the precipitated product. The final L-DOPA concentration reaches higher than 120 g/l with dicatechol conversion more than 96% in a 15-l stirred tank, demonstrating the great potential of Fn-TPL for industrial production of L-DOPA. Optimization of reaction conditions and upscaling of the recombinant enzyme TPL from Fusobacterium nucleatum in enzyme-expressing Escherichia coli cells, detailed overview | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.2 | L-tyrosine + H2O | Fusobacterium nucleatum subsp. nucleatum | - |
phenol + pyruvate + NH3 | - |
r | |
4.1.99.2 | L-tyrosine + H2O | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | - |
phenol + pyruvate + NH3 | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.2 | Fusobacterium nucleatum subsp. nucleatum | Q8RHM6 | - |
- |
4.1.99.2 | Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | Q8RHM6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.99.2 | recombinant enzyme from Escherichia coli strain BL21(DE3) | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.2 | L-tyrosine + H2O | - |
Fusobacterium nucleatum subsp. nucleatum | phenol + pyruvate + NH3 | - |
r | |
4.1.99.2 | L-tyrosine + H2O | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | phenol + pyruvate + NH3 | - |
r | |
4.1.99.2 | pyrocatechol + pyruvate + NH4+ | - |
Fusobacterium nucleatum subsp. nucleatum | L-DOPA + H2O | - |
r | |
4.1.99.2 | pyrocatechol + pyruvate + NH4+ | - |
Fusobacterium nucleatum subsp. nucleatum ATCC 25586 / CIP 101130 / JCM 8532 / LMG 13131 | L-DOPA + H2O | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.2 | Fn-TPL | - |
Fusobacterium nucleatum subsp. nucleatum |
4.1.99.2 | TnaA | - |
Fusobacterium nucleatum subsp. nucleatum |
4.1.99.2 | TPL | - |
Fusobacterium nucleatum subsp. nucleatum |
4.1.99.2 | tyrosine phenol lyase | - |
Fusobacterium nucleatum subsp. nucleatum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 15 | - |
recombinant enzyme, L-DOPA synthesis in recombinant Escherichia coli cells | Fusobacterium nucleatum subsp. nucleatum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.99.2 | 8 | - |
recombinant enzyme, L-DOPA synthesis in recombinant Escherichia coli cells | Fusobacterium nucleatum subsp. nucleatum |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
4.1.99.2 | pyridoxal 5'-phosphate | dependent on | Fusobacterium nucleatum subsp. nucleatum |