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Literature summary extracted from

  • Jiang, Y.; Liu, W.; Cheng, T.; Cao, Y.; Zhang, R.; Xian, M.
    Characterization of D-xylonate dehydratase YjhG from Escherichia coli (2015), Bioengineered, 6, 227-232 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.82 2-mercaptoethanol 1 mM, 44% loss of activity Escherichia coli
4.2.1.82 dithiothreitol 1 mM, 70% loss of activity Escherichia coli
4.2.1.82 Ni2+ 5 mM Escherichia coli
4.2.1.82 Zn2+ 5 mM Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.82 4.88
-
 D-xylonate pH 8.0, 30°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.82 Mg2+ 5 mM, required for activity, activates Escherichia coli
4.2.1.82 Mn2+ 5 mM, required for activity, activates Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.82 70000
-
 SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.82 D-xylonate Escherichia coli
-
 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.82 D-xylonate Escherichia coli K12
-
 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.82 Escherichia coli P39358
-
-
4.2.1.82 Escherichia coli K12 P39358
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.82
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.82 D-xylonate
-
 Escherichia coli 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?
4.2.1.82 D-xylonate
-
 Escherichia coli K12 2-dehydro-3-deoxy-D-arabinonate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.82 YjhG
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.82 30
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.82 20 42 20°C: about 60% of maximal activity, 42°C: about 50% of maximal activity, activity is almost undetectable above 60x01C° Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.82 70
-
 the enzyme is denatured to precipitation above 70°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.82 8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.1.82 6.7 8.5 pH 6.7: about 65% of maximal activity, pH 8.5: about 65% of maximal activity Escherichia coli