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Literature summary extracted from

  • Kulikova, V.; Morozova, E.; Rodionov, A.; Koval, V.; Anufrieva, N.; Revtovich, S.; Demidkina, T.
    Non-stereoselective decomposition of (+-)-S-alk(en)yl-l-cysteine sulfoxides to antibacterial thiosulfinates catalyzed by C115H mutant methionine gamma-lyase from Citrobacter freundii (2018), Biochimie, 151, 42-44 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.4 expression in Escherichia coli Citrobacter freundii
4.4.1.11 expression in Escherichia coli Citrobacter freundii

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.4 C115H mutant cleaves S-alk(en)yl-L-cysteine sulfoxides more effectively than the wild type Citrobacter freundii
4.4.1.11 C115H mutant cleaves S-alk(en)yl-L-cysteine sulfoxides more effectively than the wild type, reaction of EC 4.4.1.4 Citrobacter freundii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.4 0.47
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 0.54
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 0.57
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 1.1
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 1.2
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 1.26
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 1.4
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.4 Citrobacter freundii Q84AR1 cf. EC 4.4.1.11
-
4.4.1.11 Citrobacter freundii Q84AR1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.4 (+)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
4.4.1.4 (+)-methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+)-propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+-)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
4.4.1.4 (+-)-ethiin + H2O
-
 Citrobacter freundii ethanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+-)-methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 (+-)-propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.4 additional information enzyme additionally catalyzes the beta-elimination reaction of (+-)-S-alk(en)yl-L-cysteine sulfoxides to yield thiosulfinate, reaction of EC 4.4.1.4. It can decompose both diastereomers equally Citrobacter freundii ?
-
 ?
4.4.1.4 S-butyl-L-cysteine + H2O
-
 Citrobacter freundii butanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.4 S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.4 S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.4 S-propyl-L-cysteine + H2O
-
 Citrobacter freundii propanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 (+)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
4.4.1.11 (+)methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 (+)propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 (+-)-alliin + H2O
-
 Citrobacter freundii allicin + pyruvate + NH3
-
 ?
4.4.1.11 (+-)-butiin + H2O
-
 Citrobacter freundii butane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 (+-)-ethiin + H2O
-
 Citrobacter freundii ethanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 (+-)methiin + H2O
-
 Citrobacter freundii methanesulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 (+-)propiin + H2O
-
 Citrobacter freundii propane-1-sulfenic acid + pyruvate + NH3
-
 ?
4.4.1.11 additional information enzyme additionally catalyzes the beta-elimination reaction of (+-)-S-alk(en)yl-L-cysteine sulfoxides to yield thiosulfinate, reaction of EC 4.4.1.4. It can decompose both diastereomers equally Citrobacter freundii ?
-
 ?
4.4.1.11 S-butyl-L-cysteine + H2O
-
 Citrobacter freundii butanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-ethyl-L-cysteine + H2O
-
 Citrobacter freundii ethanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-methyl-L-cysteine + H2O
-
 Citrobacter freundii methanethiol + NH3 + 2-oxopropanoate
-
 ?
4.4.1.11 S-propyl-L-cysteine + H2O
-
 Citrobacter freundii propanethiol + NH3 + 2-oxopropanoate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.4 5.14
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 5.2
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 5.59
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 12.4
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 13.7
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 20.2
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 21.8
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.4.1.4 9
-
 (+-)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 9.6
-
 (+)-methiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 9.8
-
 (+-)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 11
-
 (+)-alliin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 12
-
 (+-)-ethiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 16
-
 (+)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii
4.4.1.4 18
-
 (+-)-propiin mutant C115H, pH pH 8.0, 30°C Citrobacter freundii