Literature summary extracted from
Waugh, B.; Sen, U.; Banerjee, R.
Crystal structure of phosphoglucomutase from Leishmania major at 3.5 A resolution (2016), Biochimie, 121, 102-111 .
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
alpha-D-Glucose 1,6-bisphosphate |
2fold increase of activity at 0.0001 mM |
Leishmania major |
|
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
5.4.2.2 |
hanging drop vapor diffusion method, using 0.1 M Tris-HCl, pH 8.0, 10% (w/v) PEG 3350, 10 mM MgCl2 and 0.02% (w/v) NaN3 |
Leishmania major |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
0.02135 |
- |
alpha-D-glucose 1-phosphate |
pH and temperature not specified in the publication |
Leishmania major |
|
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
Leishmania major |
- |
D-glucose 6-phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
5.4.2.2 |
Leishmania major |
Q4QCF1 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
5.4.2.2 |
Ni-NTA column chromatography |
Leishmania major |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
5.4.2.2 |
alpha-D-glucose 1-phosphate |
- |
Leishmania major |
D-glucose 6-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
5.4.2.2 |
? |
x * 63896, calculated from amino acid sequence |
Leishmania major |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
5.4.2.2 |
PGM |
- |
Leishmania major |
kcat/KM [mM/s]
EC Number |
kcat/KM Value [1/mMs-1] |
kcat/KM Value Maximum [1/mMs-1] |
Substrate |
Comment |
Organism |
Structure |
---|
5.4.2.2 |
45 |
- |
alpha-D-glucose 1-phosphate |
pH and temperature not specified in the publication |
Leishmania major |
|