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Literature summary extracted from

  • Liening, S.; Scriba, G.K.; Rummler, S.; Weinigel, C.; Kleinschmidt, T.K.; Haeggstroem, J.Z.; Werz, O.; Garscha, U.
    Development of smart cell-free and cell-based assay systems for investigation of leukotriene C4 synthase activity and evaluation of inhibitors (2016), Biochim. Biophys. Acta, 1861, 1605-1613 .
    View publication on PubMed

Application

EC Number Application Comment Organism
4.4.1.20 analysis cell-free and cell-based assay systems based on in situ-generated LTA4 that allow studying LTC4S activity and investigating LTC4S inhibitors Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.20 transfection of HEK 293 cell Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.4.1.20 5-[5-[(4-chlorophenyl)(methyl)amino]pyridine-2-carbonyl]-2-(4-methoxybenzoyl)benzoic acid tandem benzophenone amino pyridine inhibitor Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.20 Homo sapiens Q16873
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.4.1.20 commercial preparation
-
 Homo sapiens
-

Synonyms

EC Number Synonyms Comment Organism
4.4.1.20 LTC4 synthase
-
 Homo sapiens
4.4.1.20 LTC4S
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
4.4.1.20 0.000017
-
 cell-free assay, the substrate LTA4 is produced in situ, 37°C, pH not specified in the publication Homo sapiens 5-[5-[(4-chlorophenyl)(methyl)amino]pyridine-2-carbonyl]-2-(4-methoxybenzoyl)benzoic acid

General Information

EC Number General Information Comment Organism
4.4.1.20 physiological function transcellular LTC4 biosynthesis takes place between neutrophils or 5-lipoxygenase-expressing HEK293 cells that produce LTA4 from arachidonic acid and HEK293 cells expressing LTC4S that transform LTA4 to LTC4 Homo sapiens