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Literature summary extracted from

  • Thoden, J.; Vinogradov, E.; Gilbert, M.; Salinger, A.; Holden, H.
    Bacterial sugar 3,4-ketoisomerases structural insight into product stereochemistry (2015), Biochemistry, 54, 4495-4506 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.2.3 expressed in Escherichia coli Rosetta2(DE3) cells Aneurinibacillus thermoaerophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.2.3 hanging drop vapor diffusion method, using 20% poly(ethylene glycol) 8000, 200 mM NaCl, and 100 mM MOPS (pH 7.0) Aneurinibacillus thermoaerophilus

Protein Variants

EC Number Protein Variants Comment Organism
5.3.2.3 H95R the mutant shows increased activity compared to the wild type enzyme Aneurinibacillus thermoaerophilus
5.3.2.3 R15Y the mutant shows reduced activity compared to the wild type enzyme Aneurinibacillus thermoaerophilus
5.3.2.3 R15Y/H95R the mutant shows reduced activity compared to the wild type enzyme Aneurinibacillus thermoaerophilus
5.3.2.3 Y35F the mutant shows about wild type activity Aneurinibacillus thermoaerophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.2.3 0.045
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.15
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme Y35F, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.37
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y/H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.41
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.5
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose wild type enzyme, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus

Organism

EC Number Organism UniProt Comment Textmining
5.3.2.3 Aneurinibacillus thermoaerophilus Q6T1W8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.2.3 Ni-NTA column chromatography Aneurinibacillus thermoaerophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.2.3 dTDP-4-dehydro-6-deoxy-alpha-D-glucose
-
 Aneurinibacillus thermoaerophilus dTDP-3-dehydro-6-deoxy-alpha-D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.3.2.3 FdtA
-
 Aneurinibacillus thermoaerophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.2.3 0.18
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y/H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.19
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 1.23
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 3.3
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme Y35F, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 10.5
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose wild type enzyme, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.2.3 0.46
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 0.49
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme R15Y/H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 21
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose wild type enzyme, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 22
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme Y35F, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus
5.3.2.3 27
-
 dTDP-4-dehydro-6-deoxy-alpha-D-glucose mutant enzyme H95R, at pH 7.5 and 37°C Aneurinibacillus thermoaerophilus