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Literature summary extracted from

  • Bouvier, J.; Groninger-Poe, F.; Vetting, M.; Almo, S.; Gerlt, J.
    Galactaro delta-lactone isomerase Lactone isomerization by a member of the amidohydrolase superfamily (2014), Biochemistry, 53, 614-616 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
5.4.1.4 N240D the substitution produces a 28fold decrease in activity Agrobacterium tumefaciens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.1.4 additional information the presence of Ca2+, Co2+, Mg2+, Mn2+, Ni2+, or Zn2+ does not increase the rate of the enzymatic reaction Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.4.1.4 D-galactaro-1,5-lactone Agrobacterium tumefaciens
-
 D-galactaro-1,4-lactone
-
 ?
5.4.1.4 D-galactaro-1,5-lactone Agrobacterium tumefaciens C58
-
 D-galactaro-1,4-lactone
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.4.1.4 Agrobacterium tumefaciens A9CEQ7
-
-
5.4.1.4 Agrobacterium tumefaciens C58 A9CEQ7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.1.4 D-galactaro-1,5-lactone
-
 Agrobacterium tumefaciens D-galactaro-1,4-lactone
-
 ?
5.4.1.4 D-galactaro-1,5-lactone
-
 Agrobacterium tumefaciens C58 D-galactaro-1,4-lactone
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.4.1.4 D-galactarolactone isomerase
-
 Agrobacterium tumefaciens
5.4.1.4 galactaro delta-lactone isomerase
-
 Agrobacterium tumefaciens
5.4.1.4 GLI
-
 Agrobacterium tumefaciens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.4.1.4 16
-
 D-galactaro-1,5-lactone mutant enzyme N240D, pH and temperature not specified in the publication Agrobacterium tumefaciens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.4.1.4 3.6
-
 D-galactaro-1,5-lactone mutant enzyme N240D, pH and temperature not specified in the publication Agrobacterium tumefaciens