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Literature summary extracted from

  • Waugh, M.W.; Marsh, E.N.
    Solvent isotope effects on alkane formation by cyanobacterial aldehyde deformylating oxygenase and their mechanistic implications (2014), Biochemistry, 53, 5537-5543 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.5 gene PMT_1231, recombinant expression in Escherichia coli Prochlorococcus marinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.99.5 additional information
-
 additional information the rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction Prochlorococcus marinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.99.5 Fe2+ essential, di-iron center Prochlorococcus marinus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus
-
 an alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+ Prochlorococcus marinus MIT9313
-
 an alkane + formate + H2O + 2 NADP+
-
 ?

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
4.1.99.5 additional information solvent isotope effects on alkane formation by the enzyme have mechanistic implications Prochlorococcus marinus

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.5 Prochlorococcus marinus Q7V6D4
-
-
4.1.99.5 Prochlorococcus marinus MIT9313 Q7V6D4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.99.5 recombinant enzyme from Escherichia coli Prochlorococcus marinus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.1.99.5 octadecanal + O2 + 2 NADPH + 2 H+ = heptadecane + formate + H2O + 2 NADP+ proposed mechanism for deformylation of aldehydes by cADO, overview. The rate of alkane formation is the same in D2O or H2O, implying that proton transfer is not a kinetically significant step. When the ratio of protium to deuterium in the product alkane is measured as a function of the mole fraction of D2O, a D2OSIEobs of 2.19 is observed. The SIE is invariant with the mole fraction of D2O, indicating the involvement of a single protic site in the reaction. An iron-bound water molecule is the proton donor to the alkane in the reaction Prochlorococcus marinus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus an alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 a long-chain aldehyde + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus MIT9313 an alkane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 additional information enzyme assay in anaerobic conditions, quantification of hydrocarbon products by GC-MS Prochlorococcus marinus ?
-
 ?
4.1.99.5 additional information enzyme assay in anaerobic conditions, quantification of hydrocarbon products by GC-MS Prochlorococcus marinus MIT9313 ?
-
 ?
4.1.99.5 n-octadecanal + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus heptadecane + formate + H2O + 2 NADP+
-
 ?
4.1.99.5 n-octadecanal + O2 + 2 NADPH + 2 H+
-
 Prochlorococcus marinus MIT9313 heptadecane + formate + H2O + 2 NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.99.5 cyanobacterial aldehyde deformylating oxygenase
-
 Prochlorococcus marinus
4.1.99.5 PMT_1231
-
 Prochlorococcus marinus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.5 37
-
 assay at Prochlorococcus marinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.99.5 6.8
-
-
 Prochlorococcus marinus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.99.5 NADPH
-
 Prochlorococcus marinus

General Information

EC Number General Information Comment Organism
4.1.99.5 additional information solvent isotope effects on alkane formation by cyanobacterial aldehyde deformylating oxygenase and their mechanistic implications, overview Prochlorococcus marinus