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Literature summary extracted from

  • Adams, N.B.; Marklew, C.J.; Qian, P.; Brindley, A.A.; Davison, P.A.; Bullough, P.A.; Hunter, C.N.
    Structural and functional consequences of removing the N-terminal domain from the magnesium chelatase ChlH subunit of Thermosynechococcus elongatus (2014), Biochem. J., 464, 315-322 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.6.1.1 GUN4 protein stimulates enzyme activity significantly especially at low Mg2+ concentrations Thermosynechococcus vestitus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.6.1.1 expressed in Escherichia coli Rosetta 2 pLysS cells Thermosynechococcus vestitus

Protein Variants

EC Number Protein Variants Comment Organism
6.6.1.1 DELTAN159ChlH the mutant retains the ability to bind protoporphyrin and the Gun4 enhancer protein, although the addition of I and D subunits yields an extremely impaired active enzyme complex Thermosynechococcus vestitus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.6.1.1 Mg2+ required Thermosynechococcus vestitus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O Thermosynechococcus vestitus
-
 ADP + phosphate + Mg-protoporphyrin IX + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.6.1.1 Thermosynechococcus vestitus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.6.1.1 immobilized metal affinity chromatography, Q-Sepharose column chromatography, and Superdex 200 gel filtration Thermosynechococcus vestitus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.6.1.1 ATP + protoporphyrin IX + Mg2+ + H2O
-
 Thermosynechococcus vestitus ADP + phosphate + Mg-protoporphyrin IX + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
6.6.1.1 heterotrimer 1 * 150000 + 1 * 70000 + 1 * 40000, SDS-PAGE Thermosynechococcus vestitus

Synonyms

EC Number Synonyms Comment Organism
6.6.1.1 ChlH subunit Thermosynechococcus vestitus
6.6.1.1 MGCH
-
 Thermosynechococcus vestitus

Cofactor

EC Number Cofactor Comment Organism Structure
6.6.1.1 ATP
-
 Thermosynechococcus vestitus