EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.99.9 | gene ugmA, recombinant expression of His8-tagged enzyme in Escherichia coli strain BL21(DE3) | Aspergillus fumigatus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
5.4.99.9 | flavopiridol | a compound that increases the melting temperature of Aspergillus fumigatus UGM. Flavopiridol also is a non-competitive inhibitor of UGM, and docking studies reveal that flavopiriol binds in the adenine-galactopyranose pocket and interacts with several residues. This compound does not inhibit the prokaryotic UGM from Mycobacteria tuberculosis. Inhibition mechanism of flavopiriodol | Aspergillus fumigatus | |
5.4.99.9 | additional information | identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay, docking studies | Aspergillus fumigatus | |
5.4.99.9 | additional information | identification of eukaryotic UDP-galactopyranose mutase inhibitors using the ThermoFAD assay. Flavopiridol does not inhibit the prokaryotic UGM from Mycobacterium tuberculosis, in contrast to the eukaryotic enzyme from Aspergillus fumigatus | Mycobacterium tuberculosis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.9 | UDP-alpha-D-galactopyranose | Mycobacterium tuberculosis | - |
UDP-alpha-D-galactofuranose | - |
r | |
5.4.99.9 | UDP-alpha-D-galactopyranose | Aspergillus fumigatus | - |
UDP-alpha-D-galactofuranose | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.99.9 | Aspergillus fumigatus | Q4W1X2 | i.e. Neosartorya fumigata | - |
5.4.99.9 | Mycobacterium tuberculosis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.4.99.9 | recombinant His8-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, tag cleavage with TEV protease, dialysis, and another step of nickel affinity chromatography and dialysis | Aspergillus fumigatus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.99.9 | UDP-alpha-D-galactopyranose | - |
Mycobacterium tuberculosis | UDP-alpha-D-galactofuranose | - |
r | |
5.4.99.9 | UDP-alpha-D-galactopyranose | - |
Aspergillus fumigatus | UDP-alpha-D-galactofuranose | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.99.9 | AfUGM | - |
Aspergillus fumigatus |
5.4.99.9 | MtUGM | - |
Mycobacterium tuberculosis |
5.4.99.9 | UDP-Galp mutase | - |
Mycobacterium tuberculosis |
5.4.99.9 | UDP-Galp mutase | - |
Aspergillus fumigatus |
5.4.99.9 | UGM | - |
Mycobacterium tuberculosis |
5.4.99.9 | UGM | - |
Aspergillus fumigatus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.99.9 | 25 | - |
assay at | Aspergillus fumigatus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.99.9 | 7 | - |
assay at | Aspergillus fumigatus |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.99.9 | 0.454 | - |
flavopiridol | pH 7.0, 25°C, recombinant detagged enzyme | Aspergillus fumigatus |