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Literature summary extracted from

  • Lee, S.; Kim, K.H.; Kim, H.Y.; Choi, I.G.
    Crystal structure analysis of 3,6-anhydro-L-galactonate cycloisomerase suggests emergence of novel substrate specificity in the enolase superfamily (2017), Biochem. Biophys. Res. Commun., 491, 217-222 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.5.1.25 expressed in Escherichia coli BL21(DE3) cells Vibrio sp. EJY3

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.5.1.25 sitting drop vapor diffusion method, using 0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate (pH 6.5), and 20% (w/v) polyethylene glycol 8000 Vibrio sp. EJY3

Protein Variants

EC Number Protein Variants Comment Organism
5.5.1.25 H300N inactive Vibrio sp. EJY3
5.5.1.25 K169A inactive Vibrio sp. EJY3

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.5.1.25 3,6-anhydro-L-galactonate Vibrio sp. EJY3
-
 2-dehydro-3-deoxy-L-galactonate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.5.1.25 Vibrio sp. EJY3 H2IFX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.25 HisTag affinity column chromatography and Hitrap Q column chromatography Vibrio sp. EJY3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.5.1.25 3,6-anhydro-L-galactonate
-
 Vibrio sp. EJY3 2-dehydro-3-deoxy-L-galactonate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.5.1.25 ACI
-
 Vibrio sp. EJY3