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Literature summary extracted from

  • Yu, C.K.; Wang, C.J.; Chew, Y.; Wang, P.C.; Yin, H.S.; Kao, M.C.
    Functional characterization of Helicobacter pylori 26695 sedoheptulose 7-phosphate isomerase encoded by hp0857 and its association with lipopolysaccharide biosynthesis and adhesion (2016), Biochem. Biophys. Res. Commun., 477, 794-800 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.1.28 expression in Escherichia coli Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.3.1.28 0.5
-
 D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.3.1.28 40500
-
 gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
5.3.1.28 Helicobacter pylori O25528
-
-
5.3.1.28 Helicobacter pylori 26695 O25528
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.1.28 D-sedoheptulose 7-phosphate
-
 Helicobacter pylori D-glycero-D-manno-heptose 7-phosphate
-
 ?
5.3.1.28 D-sedoheptulose 7-phosphate
-
 Helicobacter pylori 26695 D-glycero-D-manno-heptose 7-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.1.28 dimer 2 * 24000, SDS-PAGE, 2 * 23100, calculated from sequence, 2 * 23200, mass spectrometry Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
5.3.1.28 GmhA
-
 Helicobacter pylori
5.3.1.28 HP0857
-
 Helicobacter pylori

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
5.3.1.28 33.1
-
 D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori

General Information

EC Number General Information Comment Organism
5.3.1.28 physiological function a knockout mutant exhibits a decreased growth rate and displays a deep rough type of lipopolysaccharide structure. It has a slight decrease in its motility and is more susceptible to novobiocin and detergents Triton X-100 and SDS. The adhesive capacity of the mutant to AGS cells is reduced significantly, and most of the infected cells don't show a classic hummingbird phenotype Helicobacter pylori

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
5.3.1.28 71.9
-
 D-sedoheptulose 7-phosphate pH not specified in the publication, temperature not specified in the publication Helicobacter pylori