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Literature summary extracted from

  • Zhu, Q.; Liu, X.
    Characterization of non-heme iron aliphatic halogenase WelO5* from Hapalosiphon welwitschii IC-52-3 Identification of a minimal protein sequence motif that confers enzymatic chlorination specificity in the biosynthesis (2017), Beilstein J. Org. Chem., 13, 1168-1173 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.20.14 heterologous expressions as a N-terminal hepta-His-tagged enzyme in Escherichia coli Hapalosiphon welwitschii

Protein Variants

EC Number Protein Variants Comment Organism
1.14.20.14 additional information a WelO5-variant is constructed by swapping the residues 215-232 of WelO5 from Hapalosiphon welwitschii UTEX B1830 to those of WelO5* of Hapalosiphon welwitschii IC-52-3. The variant displays a noticeably enhanced activity towards 12-epi-hapalindole C. The activity of WelO5-var towards 12-epi-hapalindole C is elevated to a comparable level as the wild type WelO5* from Hapalosiphon welwitschii IC-52-3, while retaining its fidelity towards 12-epi-fischerindole U Hapalosiphon welwitschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.20.14 Fe2+ iron(II)-dependent enzyme Hapalosiphon welwitschii

Organism

EC Number Organism UniProt Comment Textmining
1.14.20.14 Hapalosiphon welwitschii
-
-
-
1.14.20.14 Hapalosiphon welwitschii A0A067YX61
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-
1.14.20.14 Hapalosiphon welwitschii IC-52-3
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-
-
1.14.20.14 Hapalosiphon welwitschii UTEX B 1830 A0A067YX61
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-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.20.14
-
 Hapalosiphon welwitschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.20.14 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epi-fischerindole U as WelO5 Hapalosiphon welwitschii 12-epi-fischerindole G + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epifischerindole U as WelO5 Hapalosiphon welwitschii 12-epi-fischerindole G + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epifischerindole U as WelO5 Hapalosiphon welwitschii UTEX B 1830 12-epi-fischerindole G + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epi-fischerindole U as WelO5 Hapalosiphon welwitschii IC-52-3 12-epi-fischerindole G + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epi-fischerindole U as WelO5 Hapalosiphon welwitschii 12-epi-hapalindole E + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epifischerindole U as WelO5 Hapalosiphon welwitschii 12-epi-hapalindole E + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epifischerindole U as WelO5 Hapalosiphon welwitschii UTEX B 1830 12-epi-hapalindole E + succinate + CO2 + H2O
-
 ?
1.14.20.14 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride WelO5* from Hapalosiphon welwitschii IC-52-3 exhibits an enhanced specificity towards 12-epi-hapalindole C, a substrate poorly processed by WelO5 from Hapalosiphon welwitschii UTEX B1830, while maintaining its fidelity towards 12-epi-fischerindole U as WelO5 Hapalosiphon welwitschii IC-52-3 12-epi-hapalindole E + succinate + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.20.14 welO5
-
 Hapalosiphon welwitschii
1.14.20.14 WelO5*
-
 Hapalosiphon welwitschii