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Literature summary extracted from

  • Spiess, E.; Goerisch, H.
    Purification and characterization of chlorobenzene cis-dihydrodiol dehydrogenase from Xanthobacter flavus 14p1 (1996), Arch. Microbiol., 165, 201-205 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.119 8-hydroxyquinoline 1 mM, 54% inhibition Xanthobacter flavus
1.3.1.119 HgCl2 0.1 mM, complete loss of activity, most potent inhibitor Xanthobacter flavus
1.3.1.119 KCN 1 mM, 26% inhibition Xanthobacter flavus
1.3.1.119 additional information no inhibition by EDTA, 2,2-dipyridyl, and 1,10-phenanthroline Xanthobacter flavus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.119 0.005
-
 NAD+ pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol Xanthobacter flavus
1.3.1.119 0.02
-
 NAD+ pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol Xanthobacter flavus
1.3.1.119 0.21
-
 3,6-dichlorobenzene cis-dihydrodiol pH 8.0, 30°C Xanthobacter flavus
1.3.1.119 0.78
-
 benzene cis-dihydrodiol pH 8.0, 30°C Xanthobacter flavus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.119 110000
-
 gel filtration Xanthobacter flavus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.119 Xanthobacter flavus
-
-
-
1.3.1.119 Xanthobacter flavus DSM 10330
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.119
-
 Xanthobacter flavus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.119 32
-
 substrate: 3,6-dichlorobenzene cis-dihydrodiol, pH 8.0, 30°C Xanthobacter flavus
1.3.1.119 63
-
 substrate: benzene cis-dihydrodiol, pH 8.0, 30°C Xanthobacter flavus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.119 3,4-dichlorobenzene cis-dihydrodiol + NAD+ activity is 30% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3,4-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,4-dichlorobenzene cis-dihydrodiol + NAD+ activity is 30% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus DSM 10330 3,4-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,5-dichlorobenzene cis-dihydrodiol + NAD+ activity is 195% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3,5-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,5-dichlorobenzene cis-dihydrodiol + NAD+ activity is 195% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus DSM 10330 3,5-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,6-dichlorobenzene cis-dihydrodiol + NAD+ activity is 60% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3,6-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,6-dichlorobenzene cis-dihydrodiol + NAD+ activity is 60% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus DSM 10330 3,6-dichlorocatechol + NADH + H+
-
 ?
1.3.1.119 3,6-dimethylbenzene cis-dihydrodiol + NAD+ activity is 105% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3,6-dimethylcatechol + NADH + H+
-
 ?
1.3.1.119 3,6-dimethylbenzene cis-dihydrodiol + NAD+ activity is 105% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus DSM 10330 3,6-dimethylcatechol + NADH + H+
-
 ?
1.3.1.119 3-chlorobenzene cis-dihydrodiol + NAD+ activity is 170% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3-chlorocatechol + NADH + H+
-
 ?
1.3.1.119 3-chlorobenzene cis-dihydrodiol + NAD+ activity is 170% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus DSM 10330 3-chlorocatechol + NADH + H+
-
 ?
1.3.1.119 3-fluorobenzene cis-dihydrodiol + NAD+ activity is 145% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3-fluorocatechol + NADH + H+
-
 ?
1.3.1.119 3-methylbenzene cis-dihydrodiol + NAD+ activity is 185% compared to the activity with benzene cis-dihydrodiol Xanthobacter flavus 3-methylcatechol + NADH + H+
-
 ?
1.3.1.119 benzene cis-dihydrodiol + NAD+
-
 Xanthobacter flavus catechol + NADH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.119 homotetramer 4 * 26500, SDS-PAGE Xanthobacter flavus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.119 3,6-dichlorobenzene DDH
-
 Xanthobacter flavus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.119 14.5
-
 3,6-dichlorobenzene cis-dihydrodiol pH 8.0, 30°C Xanthobacter flavus
1.3.1.119 14.5
-
 NAD+ pH 8.0, 30°C, cosubstrate: 3,6-dichlorobenzene cis-dihydrodiol Xanthobacter flavus
1.3.1.119 27.8
-
 benzene cis-dihydrodiol pH 8.0, 30°C Xanthobacter flavus
1.3.1.119 27.8
-
 NAD+ pH 8.0, 30°C, cosubstrate: benzene cis-dihydrodiol Xanthobacter flavus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.3.1.119 6
-
 optimum stability at 4°C Xanthobacter flavus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.119 NAD+ the enzyme accepts also NADP+ as cosubstrate. The activity with NADP+ is reduced to 14% of that with NAD+ Xanthobacter flavus
1.3.1.119 NADP+ the activity with NADP+ is reduced to 14% of that with NAD+ Xanthobacter flavus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.3.1.119 Xanthobacter flavus
-
-
 5.4