Literature summary extracted from

Oroz-Guinea, I.; Sanchez-Moreno, I.; Mena, M.; Garcia-Junceda, E.
Hyperthermophilic aldolases as biocatalyst for C-C bond formation rhamnulose 1-phosphate aldolase from Thermotoga maritima (2015), Appl. Microbiol. Biotechnol., 99, 3057-3068 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
4.1.2.19	expressed in Escherichia coli BL21(DE3) cells	Thermotoga maritima

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
4.1.2.19	3.6	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
4.1.2.19	Co2+	maximum activity at 0.1875 mM	Thermotoga maritima

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
4.1.2.19	114000	-	gel filtration	Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
4.1.2.19	Glycerone phosphate + (S)-lactaldehyde	Thermotoga maritima	-	L-Rhamnulose 1-phosphate	-	r
4.1.2.19	L-Rhamnulose 1-phosphate	Thermotoga maritima	-	Glycerone phosphate + (S)-lactaldehyde	-	r

Organic Solvent Stability

EC Number	Organic Solvent	Comment	Organism
4.1.2.19	acetonitrile	the enzyme retains 90 % of its activity in the presence of 40 % (v/v) of acetonitrile	Thermotoga maritima

Organism

EC Number	Organism	UniProt	Comment	Textmining
4.1.2.19	Thermotoga maritima	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
4.1.2.19	Ni-IDA agarose column chromatography	Thermotoga maritima

Storage Stability

EC Number	Storage Stability	Organism
4.1.2.19	25°C, 28 days, about 30% loss of activity	Thermotoga maritima
4.1.2.19	4°C, 28 days, less than 20% loss of activity	Thermotoga maritima

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4.1.2.19	Glycerone phosphate + (S)-lactaldehyde	-	Thermotoga maritima	L-Rhamnulose 1-phosphate	-	r
4.1.2.19	Glycerone phosphate + (S)-lactaldehyde	more than 90% aldol formation at 45°C	Thermotoga maritima	L-Rhamnulose 1-phosphate	-	r
4.1.2.19	glycerone phosphate + benzyloxyacetaldehyde	68% aldol formation at 45°C	Thermotoga maritima	(3R,4S)-5-(benzyloxy)-3,4-dihydroxy-2-oxopentyl phosphate	-	?
4.1.2.19	glycerone phosphate + ethyl-3-methyl-4-oxocrotonate	87% aldol formation at 45°C	Thermotoga maritima	(3R,4S,5E)-7-ethoxy-3,4-dihydroxy-5-methyl-2,7-dioxohept-5-en-1-yl phosphate	-	?
4.1.2.19	glycerone phosphate + phenylacetaldehyde	53% aldol formation at 45°C	Thermotoga maritima	(3R,4S)-3,4-dihydroxy-2-oxo-5-phenylpentyl phosphate	-	?
4.1.2.19	L-Rhamnulose 1-phosphate	-	Thermotoga maritima	Glycerone phosphate + (S)-lactaldehyde	-	r

Subunits

EC Number	Subunits	Comment	Organism
4.1.2.19	homotetramer	4 * 29000, SDS-PAGE	Thermotoga maritima
4.1.2.19	homotetramer	4 * 28900, calculated from amino acid sequence	Thermotoga maritima

Synonyms

EC Number	Synonyms	Comment	Organism
4.1.2.19	Rha-1PA	-	Thermotoga maritima
4.1.2.19	TM1072	-	Thermotoga maritima

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
4.1.2.19	95	-	-	Thermotoga maritima

Temperature Stability [°C]

EC Number	Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
4.1.2.19	80	115	the enzyme shows a half-life of 44 h, 33 h and 3.5 h at 80°C, 95°C and 115°C, respectively	Thermotoga maritima

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
4.1.2.19	2.52	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.1.2.19	8	-	-	Thermotoga maritima

kcat/KM [mM/s]

EC Number	kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
4.1.2.19	0.7	-	L-rhamnulose 1-phosphate	at pH 8.0 and 25°C	Thermotoga maritima

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)