EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.5 | expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | Cu2+ | about 20% residual activity at 1 mM | Bacillus subtilis | |
4.1.1.5 | Fe2+ | about 60% residual activity at 1 mM | Bacillus subtilis | |
4.1.1.5 | Mn2+ | about 70% residual activity at 1 mM | Bacillus subtilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.5 | 21 | - |
DL-2-hydroxy-2-methyl-3-oxobutanoate | at pH 6.0 and 30°C | Bacillus subtilis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.5 | Ba2+ | about 110% activity at 1 mM | Bacillus subtilis | |
4.1.1.5 | Ca2+ | about 160% activity at 1 mM | Bacillus subtilis | |
4.1.1.5 | Mg2+ | about 120% activity at 1 mM | Bacillus subtilis | |
4.1.1.5 | Zn2+ | the enzyme contains a zinc ion and shows about 130% activity at 1 mM | Bacillus subtilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | (2R)-2-hydroxy-2-methyl-3-oxobutanoate | Bacillus subtilis | - |
(3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2R)-2-hydroxy-2-methyl-3-oxobutanoate | Bacillus subtilis 168 | - |
(3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Bacillus subtilis | best substrate | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2S)-2-hydroxy-2-methyl-3-oxobutanoate | Bacillus subtilis 168 | best substrate | (3R)-3-hydroxybutan-2-one + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.5 | Bacillus subtilis | - |
- |
- |
4.1.1.5 | Bacillus subtilis 168 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.5 | HiTrap DEAE column chromatography and Superdex 200 gel filtration | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.5 | (2R)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Bacillus subtilis | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2R)-2-hydroxy-2-methyl-3-oxobutanoate | - |
Bacillus subtilis 168 | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2S)-2-hydroxy-2-methyl-3-oxobutanoate | best substrate | Bacillus subtilis | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | (2S)-2-hydroxy-2-methyl-3-oxobutanoate | best substrate | Bacillus subtilis 168 | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | DL-2-hydroxy-2-methyl-3-oxobutanoate | - |
Bacillus subtilis | (3R)-3-hydroxybutan-2-one + CO2 | - |
? | |
4.1.1.5 | DL-2-hydroxy-2-methyl-3-oxobutanoate | - |
Bacillus subtilis 168 | (3R)-3-hydroxybutan-2-one + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.5 | ? | x * 28799, calculated from amino acid sequence | Bacillus subtilis |
4.1.1.5 | ? | x * 28983, MALDI-TOF mass spectrometry | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.5 | ALDC | - |
Bacillus subtilis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.5 | 2.2 | - |
DL-2-hydroxy-2-methyl-3-oxobutanoate | at pH 6.0 and 30°C | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 5 | - |
- |
Bacillus subtilis |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.5 | 4.5 | 8.5 | the enzyme activityremains stable between pH 4.5 and 8.5 | Bacillus subtilis |