EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.11 | 2-mercaptoethanol | 10 mM, 135% of initial activity | Flammeovirga sp. MY04 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.2.11 | expression in Escherichia coli | Flammeovirga sp. MY04 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.2.2.11 | additional information | a truncated protein consisting of the catalytic module degrades alginate into unsaturated oligosaccharides with maximal activity at 30°C, 10°C lower than the temperature for wild-type. Thermostability ranges from 0 to 30°C and highest activity is at pH 7.0 | Flammeovirga sp. MY04 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.2.2.11 | Co2+ | 1 mM, 116% of initial activity | Flammeovirga sp. MY04 |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.2.11 | Flammeovirga sp. MY04 | A0A0M4N5N7 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 264 | - |
substrate alginate, truncated protein consisting of the catalytic module, pH 6.0, 40°C | Flammeovirga sp. MY04 |
4.2.2.11 | 620 | - |
substrate alginate, wild-type, pH 6.0, 40°C | Flammeovirga sp. MY04 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.2.11 | alginate | - |
Flammeovirga sp. MY04 | unsaturated algino-oligosaccharides | - |
? | |
4.2.2.11 | saturated hexa((1-4)-alpha-L-guluronan) | - |
Flammeovirga sp. MY04 | saturated di((1-4)-alpha-L-guluronan) + unsaturated tetra((1-4)-alpha-L-guluronan) | - |
? | |
4.2.2.11 | saturated penta((1-4)-alpha-L-guluronan) | - |
Flammeovirga sp. MY04 | saturated di((1-4)-alpha-L-guluronan) + unsaturated tri((1-4)-alpha-L-guluronan) | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.2.2.11 | ? | x * 61000, calculated from sequence | Flammeovirga sp. MY04 |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.2.11 | AlgL5 | - |
Flammeovirga sp. MY04 |
4.2.2.11 | Aly5 | - |
Flammeovirga sp. MY04 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 40 | - |
- |
Flammeovirga sp. MY04 |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | - |
40 | 2 h, more than 80% residual activity | Flammeovirga sp. MY04 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 6 | - |
- |
Flammeovirga sp. MY04 |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
4.2.2.11 | 5 | 8 | 2 h, more than 80% residual activity | Flammeovirga sp. MY04 |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
4.2.2.11 | Flammeovirga sp. MY04 | calculated from sequence | - |
4.1 |