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Literature summary extracted from
- Identification of a specific maleate hydratase in the direct hydrolysis route of the gentisate pathway (2015), Appl. Environ. Microbiol., 81, 5753-5760 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.2.1.4 | glutathione | dependent on glutathione | Pseudomonas alcaligenes |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.31 | expression in Escherichia coli | Pseudomonas alcaligenes |
| 5.2.1.4 | overexpression in Escherichia coli | Pseudomonas alcaligenes |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.23 | 3-maleylpyruvate + H2O | Pseudomonas alcaligenes | the enzyme is involved in maleylpyruvate catabolism | maleate + pyruvate | - |
? | |
| 3.7.1.23 | 3-maleylpyruvate + H2O | Pseudomonas alcaligenes NCIMB 9867 | the enzyme is involved in maleylpyruvate catabolism | maleate + pyruvate | - |
? | |
| 4.2.1.31 | maleate + H2O | Pseudomonas alcaligenes | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway | (R)-malate | - |
? | |
| 4.2.1.31 | maleate + H2O | Pseudomonas alcaligenes NCIMB 9867 | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway | (R)-malate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.23 | Pseudomonas alcaligenes | Q0QFQ3 | - |
- |
| 3.7.1.23 | Pseudomonas alcaligenes NCIMB 9867 | Q0QFQ3 | - |
- |
| 4.2.1.31 | Pseudomonas alcaligenes | Q0QFQ7 AND Q0QFQ6 | Q0QFQ7: hbzI, Q0QFQ6: hbzJ | - |
| 4.2.1.31 | Pseudomonas alcaligenes NCIMB 9867 | Q0QFQ7 AND Q0QFQ6 | Q0QFQ7: hbzI, Q0QFQ6: hbzJ | - |
| 5.2.1.4 | Pseudomonas alcaligenes | - |
- |
- |
| 5.2.1.4 | Pseudomonas alcaligenes NCIMB 9867 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.31 | - |
Pseudomonas alcaligenes |
| 5.2.1.4 | - |
Pseudomonas alcaligenes |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.23 | 3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes | maleate + pyruvate | - |
? | |
| 3.7.1.23 | 3-maleylpyruvate + H2O | the enzyme is involved in maleylpyruvate catabolism | Pseudomonas alcaligenes | maleate + pyruvate | - |
? | |
| 3.7.1.23 | 3-maleylpyruvate + H2O | - |
Pseudomonas alcaligenes NCIMB 9867 | maleate + pyruvate | - |
? | |
| 3.7.1.23 | 3-maleylpyruvate + H2O | the enzyme is involved in maleylpyruvate catabolism | Pseudomonas alcaligenes NCIMB 9867 | maleate + pyruvate | - |
? | |
| 4.2.1.31 | maleate + H2O | - |
Pseudomonas alcaligenes | (R)-malate | - |
? | |
| 4.2.1.31 | maleate + H2O | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway | Pseudomonas alcaligenes | (R)-malate | - |
? | |
| 4.2.1.31 | maleate + H2O | - |
Pseudomonas alcaligenes NCIMB 9867 | (R)-malate | - |
? | |
| 4.2.1.31 | maleate + H2O | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway | Pseudomonas alcaligenes NCIMB 9867 | (R)-malate | - |
? | |
| 5.2.1.4 | 3-Maleylpyruvate | - |
Pseudomonas alcaligenes | 3-Fumarylpyruvate | - |
r | |
| 5.2.1.4 | 3-Maleylpyruvate | - |
Pseudomonas alcaligenes NCIMB 9867 | 3-Fumarylpyruvate | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.31 | dimer | - |
Pseudomonas alcaligenes |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.23 | hbzF | - |
Pseudomonas alcaligenes |
| 4.2.1.31 | HbzIJ | - |
Pseudomonas alcaligenes |
| 5.2.1.4 | HbzG | - |
Pseudomonas alcaligenes |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.31 | 23 | - |
assay at | Pseudomonas alcaligenes |
| 5.2.1.4 | 23 | - |
assay at | Pseudomonas alcaligenes |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.31 | 7.4 | - |
assay at | Pseudomonas alcaligenes |
| 5.2.1.4 | 7.4 | - |
assay at | Pseudomonas alcaligenes |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.7.1.23 | malfunction | hbzF-disrupted mutant could still grow on gentisate | Pseudomonas alcaligenes |
| 3.7.1.23 | metabolism | the enzyme is involved in maleylpyruvate catabolism | Pseudomonas alcaligenes |
| 4.2.1.31 | metabolism | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway | Pseudomonas alcaligenes |
| 5.2.1.4 | malfunction | disruption of hbzG eliminates the ability to grow on gentisate | Pseudomonas alcaligenes |
| 5.2.1.4 | metabolism | the enzyme is involved in maleylpyruvate catabolism in the gentisate pathway. The maleylpyruvate isomerization route is a complete pathway for gentisate catabolism in the mutant strain strain SponMu, whereas the direct hydrolysis route is not | Pseudomonas alcaligenes |
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