Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Witthoff, S.; Schmitz, K.; Niedenfuehr, S.; Noeh, K.; Noack, S.; Bott, M.; Marienhagen, J.
    Metabolic engineering of Corynebacterium glutamicum for methanol metabolism (2015), Appl. Environ. Microbiol., 81, 2215-2225 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
5.3.1.27 synthesis engineering of Corynebacterium glutamicum toward the utilization of methanol as an auxiliary carbon source in a sugar-based medium by heterologous expression of a methanol dehydrogenase from Bacillus methanolicus, and implementation of 3-hexulose-6-phosphate synthase and 6-phospho-3-hexuloisomerasehe of Bacillus subtilis. The recombinant strain shows an average methanol consumption rate of 1.7 mM/h in a glucose-methanol medium, and the culture grows to a higher cell density than in medium without methanol Bacillus subtilis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.43 expressed in Corynebacterium glutamicum Bacillus subtilis
4.1.2.43 expressed in Corynebacterium glutamicum Mycobacterium gastri
5.3.1.27 expression in Corynebacterium glutamicum Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.43 D-arabino-hex-3-ulose 6-phosphate Bacillus subtilis
-
 D-ribulose 5-phosphate + formaldehyde
-
 ?
4.1.2.43 D-arabino-hex-3-ulose 6-phosphate Mycobacterium gastri
-
 D-ribulose 5-phosphate + formaldehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Bacillus subtilis
-
-
-
4.1.2.43 Mycobacterium gastri
-
-
-
5.3.1.27 Bacillus subtilis P42404
-
-
5.3.1.27 Bacillus subtilis 168 P42404
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-arabino-hex-3-ulose 6-phosphate
-
 Bacillus subtilis D-ribulose 5-phosphate + formaldehyde
-
 ?
4.1.2.43 D-arabino-hex-3-ulose 6-phosphate
-
 Mycobacterium gastri D-ribulose 5-phosphate + formaldehyde
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 HPS
-
 Bacillus subtilis
4.1.2.43 HPS
-
 Mycobacterium gastri
5.3.1.27 HxlB
-
 Bacillus subtilis
5.3.1.27 YckF
-
 Bacillus subtilis