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Literature summary extracted from

  • Jacopini, S.; Vincenti, S.; Mariani, M.; Brunini-Bronzini de Caraffa, V.; Gambotti, C.; Desjobert, J.M.; Muselli, A.; Costa, J.; Tomi, F.; Berti, L.; Maury, J.
    Activation and stabilization of olive recombinant 13-hydroperoxide lyase using selected additives (2017), Appl. Biochem. Biotechnol., 182, 1000-1013 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.2.1.92 glycine best at 2.5% w/v, 28.7% activation Olea europaea
4.2.1.92 additional information for enzyme stability glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4 (0.25 M) provide the highest activation of HPL full-length activity, while the best matured HPL activity is obtained with Na2SO4 (0.25 M) and NaCl (1 M), while for enzyme activity and C6-aldehyde production, glycine, NaCl, and Na2SO4 are appropriate additives and NaCl appears to be the best additive, at least for hexanal production Olea europaea
4.2.1.92 Na2SO4 best at 0.25 M Olea europaea
4.2.1.92 NaCl best at 0.5 M Olea europaea

Application

EC Number Application Comment Organism
4.2.1.92 synthesis the recombinant 13-HPL enzyme is useful biocatalyst to produce C6-aldehydes Olea europaea

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.92 recombinant expression of His-tagged full-length enzyme or matured enzyme with truncated chloroplast transit peptide in Escherichia coli strain M15 Olea europaea

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.92 additional information optimization of recombinant full-length and truncated enzymes stabilization by additives for production of C6-aldehydes, overview Olea europaea

General Stability

EC Number General Stability Organism
4.2.1.92 for enzyme stability glycine (2.5% w/v), NaCl (0.5 M), and Na2SO4 (0.25 M) provide the highest activation of HPL full-length activity, while the best matured HPL activity is obtained with Na2SO4 (0.25 M) and NaCl (1 M), while for enzyme activity and C6-aldehyde production, glycine, NaCl, and Na2SO4 are appropriate additives and NaCl appears to be the best additive, at least for hexanal production Olea europaea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid Olea europaea
-
 hexanal + 12-oxo-9-dodecenoic acid
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid Olea europaea
-
 (Z)-3-hexenal + 12-oxo-9-dodecenoic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.92 Olea europaea G8XQN2 Leccino variety
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.92 proteolytic modification the matured enzyme lacks the chloroplast transit peptide, which is cleaved off for maturation Olea europaea

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.92 recombinant His-tagged full-length and truncated enzymes from Escherichia coli strain M15 by cobalt affinity chromatography Olea europaea

Storage Stability

EC Number Storage Stability Organism
4.2.1.92 -20°C--80°C, purified recombinant full-length and matured enzymes, 100% of the rHPL activities are maintained during 5 weeks of storage in the presence of 10% v/v glycerol (best), about 70% activity are remaining at 40% glycerol after 5 weeks. Freezing without a cryoprotectant inactivates both enzymes rapidly. The enzyme activity is greatly reduced within 3 weeks for HPL full-length and within 1 week for matured HPL since only about 20% of the enzyme activity remain when they are stored at -20°C or -80°C Olea europaea
4.2.1.92 4°C, purified recombinant full-length and matured enzymes, quite stable for 5 weeks Olea europaea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.92 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid
-
 Olea europaea hexanal + 12-oxo-9-dodecenoic acid
-
 ?
4.2.1.92 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid
-
 Olea europaea (Z)-3-hexenal + 12-oxo-9-dodecenoic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.1.92 13-HPL
-
 Olea europaea
4.2.1.92 13-hydroperoxide lyase
-
 Olea europaea
4.2.1.92 HPL
-
 Olea europaea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.92 25
-
-
 Olea europaea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.92 17.89
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant truncated enzyme Olea europaea
4.2.1.92 26.38
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant full-length enzyme Olea europaea
4.2.1.92 42
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant full-length enzyme, with 2.5% glycine w/v Olea europaea
4.2.1.92 46.9
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant truncated enzyme, with 2.5% glycine w/v Olea europaea
4.2.1.92 73.2
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant truncated enzyme, with 0.5 M NaCl Olea europaea
4.2.1.92 133.9
-
 (9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoic acid pH 7.5, 25°C, recombinant full-length enzyme, with 0.5 M NaCl Olea europaea
4.2.1.92 402.62
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant truncated enzyme Olea europaea
4.2.1.92 564.95
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant full-length enzyme Olea europaea
4.2.1.92 579
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant full-length enzyme, with 0.5 M NaCl Olea europaea
4.2.1.92 587.4
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant full-length enzyme, with 2.5% glycine w/v Olea europaea
4.2.1.92 701.6
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant truncated enzyme, with 0.5 M NaCl Olea europaea
4.2.1.92 843.3
-
 (9Z,11E,13S,15Z)-13-hydroperoxy-9,11,15-octadecatrienoic acid pH 7.5, 25°C, recombinant truncated enzyme, with 2.5% glycine w/v Olea europaea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.92 7.5
-
-
 Olea europaea

General Information

EC Number General Information Comment Organism
4.2.1.92 metabolism hydroperoxide lyase (HPL) is an enzyme found in higher plants that is involved in a lipid oxidation pathway, called the lipoxygenase pathway, which is activated in response to wounds or pathogen attacks Olea europaea
4.2.1.92 physiological function hydroperoxide lyase (HPL) is an enzyme found in higher plants that is involved in a lipid oxidation pathway, called the lipoxygenase pathway, which is activated in response to wounds or pathogen attacks. Volatile C6-aldehydes and C9-aldehydes produced by HPLs and their corresponding alcohols are known as green leaf volatiles (GLVs) and are responsible for the fresh green odor of many fruits and vegetables. GLVs such as hexanal, (Z)-3-hexenal, and (E)-2-hexenal are associated to the green note odor that is of major importance in the fragrance and flavor industries. They are also widely used in the food and beverage industry to restore the fresh green character lost during processing or to enhance food storage due to their antimicrobial activities Olea europaea