EC Number | Cloned (Comment) | Organism |
---|---|---|
1.14.20.14 | - |
Fischerella ambigua |
1.14.20.14 | - |
Hapalosiphon welwitschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.20.14 | additional information | generation of a pair of chimeric proteins by fusing the N-terminal WelO5 (aa1-145) from Hapalosiphon welwitschii UTEX B 1830 with C-terminal AmbO5 (aa146-290) from Fischerella ambigua to give WelO5AmbO5 and vice versa to give AmbO5WelO5. WelO5AmbO5 is able to convert ambiguine C to ambiguine B, albeit at a lower efficiency comparing with wild-type AmbO5, whereas AmbO5WelO5 shows no measurable activity towards ambiguine C under identical assay conditions. This result implicates the C-terminal residues of AmbO5 may play a role on its broadened substrate scope | Fischerella ambigua |
1.14.20.14 | additional information | generation of a pair of chimeric proteins by fusing the N-terminal WelO5 (aa1-145) from Hapalosiphon welwitschii UTEX B 1830 with C-terminal AmbO5 (aa146-290) from Fischerella ambigua to give WelO5AmbO5 and vice versa to give AmbO5WelO5. WelO5AmbO5 is able to convert ambiguine C to ambiguine B, albeit at a lower efficiency comparing with wild-type AmbO5, whereas AmbO5WelO5 shows no measurable activity towards ambiguine C under identical assay conditions. This result implicates the C-terminal residues of AmbO5 may play a role on its broadened substrate scope | Hapalosiphon welwitschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.20.14 | Fischerella ambigua | A0A076NA91 | - |
- |
1.14.20.14 | Hapalosiphon welwitschii | A0A067YX61 | - |
- |
1.14.20.14 | Hapalosiphon welwitschii UTEX B 1830 | A0A067YX61 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.20.14 | - |
Fischerella ambigua |
1.14.20.14 | - |
Hapalosiphon welwitschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.20.14 | 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | 12-epi-fischerindole U + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii UTEX B 1830 | 12-epi-fischerindole G + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | 12-epi-hapalindole C + 2-oxoglutarate + O2 + chloride | - |
Hapalosiphon welwitschii UTEX B 1830 | 12-epi-hapalindole E + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | ambiguine C + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine B + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | ambiguine H + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine A + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | ambiguine I + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine E + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | ambiguine L + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | ambiguine K + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | hapalindole U + 2-oxoglutarate + O2 + chloride | - |
Fischerella ambigua | hapalindole G + succinate + CO2 + H2O | - |
? | |
1.14.20.14 | additional information | no activity with hapalindole U, ambiguine H, ambiguine C, ambiguine L, ambiguine I, ambiguine E | Hapalosiphon welwitschii | ? | - |
? | |
1.14.20.14 | additional information | no activity with hapalindole U, ambiguine H, ambiguine C, ambiguine L, ambiguine I, ambiguine E | Hapalosiphon welwitschii UTEX B 1830 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.20.14 | AmbO5 protein | - |
Fischerella ambigua |
1.14.20.14 | WelO5 protein | - |
Hapalosiphon welwitschii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.20.14 | 0.21 | - |
hapalindole U | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.67 | - |
ambiguine L | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.68 | - |
ambiguine H | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.68 | - |
ambiguine I | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.7 | - |
ambiguine C | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.71 | - |
12-epi-hapalindole C | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 0.73 | - |
12-epi-hapalindole C | pH and temperature not specified in the publication | Hapalosiphon welwitschii | |
1.14.20.14 | 1.7 | - |
12-epi-fischerindole U | pH and temperature not specified in the publication | Fischerella ambigua | |
1.14.20.14 | 1.8 | - |
12-epi-fischerindole U | pH and temperature not specified in the publication | Hapalosiphon welwitschii |