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Literature summary extracted from

  • Qin, H.M.; Imai, F.L.; Miyakawa, T.; Kataoka, M.; Kitamura, N.; Urano, N.; Mori, K.; Kawabata, H.; Okai, M.; Ohtsuka, J.; Hou, F.; Nagata, K.; Shimizu, S.; Tanokura, M.
    L-allo-threonine aldolase with an H128Y/S292R mutation from Aeromonas jandaei DK-39 reveals the structural basis of changes in substrate stereoselectivity (2014), Acta Crystallogr. Sect. D, 70, 1695-1703 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.49 expression in Escherichia coli Rosetta(DE3) Aeromonas jandaei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.2.49 sitting-drop vapour-diffusion method, crystal structures of wild-type enzyme and its mutant H128Y/S292R are determined at 2.59 and 2.50 A resolution Aeromonas jandaei

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.49 H128Y 8.4fold increase in activity towards L-threonine and a 2.0fold increase towards L-allo-threonine compared with the wild-type enzyme Aeromonas jandaei
4.1.2.49 H128Y/S292R 3fold and 322fold increased kcat/Km values towards towards L-allo-threonine and L-threonine compared with the wild-type enzyme Aeromonas jandaei
4.1.2.49 S292R improved activity towards L-allo-threonine and L-threonine Aeromonas jandaei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.49 0.386
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
4.1.2.49 0.402
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 0.444
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
4.1.2.49 0.513
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 3.27
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
4.1.2.49 4.48
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 31.6
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 32.7
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.49 Aeromonas jandaei O07051
-
-
4.1.2.49 Aeromonas jandaei DK-39 O07051
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.49
-
 Aeromonas jandaei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.49 L-allo-threonine
-
 Aeromonas jandaei glycine + acetaldehyde
-
 r
4.1.2.49 L-allo-threonine
-
 Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 r
4.1.2.49 L-threonine low activity Aeromonas jandaei glycine + acetaldehyde
-
 r
4.1.2.49 L-threonine low activity Aeromonas jandaei DK-39 glycine + acetaldehyde
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.2.49 LATA
-
 Aeromonas jandaei
4.1.2.49 ltaA
-
 Aeromonas jandaei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.49 0.641
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 0.913
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
4.1.2.49 1.81
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 2.14
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
4.1.2.49 13.8
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 16.5
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 18.3
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
4.1.2.49 22
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.49 pyridoxal 5'-phosphate dependent on Aeromonas jandaei

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.1.2.49 0.00203
-
 L-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 0.0279
-
 L-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
4.1.2.49 0.404
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 0.654
-
 L-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei
4.1.2.49 26.9
-
 L-allo-threonine pH 8.0, 30°C, wild-type enzyme Aeromonas jandaei
4.1.2.49 41
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y Aeromonas jandaei
4.1.2.49 41.2
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme S292R Aeromonas jandaei
4.1.2.49 83.3
-
 L-allo-threonine pH 8.0, 30°C, mutant enzyme H128Y/S292R Aeromonas jandaei