EC Number | Cloned (Comment) | Organism |
---|---|---|
5.4.3.3 | genes kamD and kam E encode subunits alpha and beta | Acetoanaerobium sticklandii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.3.3 | additional information | - |
additional information | kinetic parameters of enzyme 5,6-LAM are measured using D-lysine or isotope-labeled D-lysine-4,4,5,5-d4 as a substrate, overview | Acetoanaerobium sticklandii | |
5.4.3.3 | 1 | - |
D-Lysine | with pyridoxal 5'-phosphate N-oxide as cofactor, pH 8.5, 37°C | Acetoanaerobium sticklandii | |
5.4.3.3 | 14.2 | - |
D-Lysine | with pyridoxal 5'-phosphate as cofactor, pH 8.5, 37°C | Acetoanaerobium sticklandii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.3.3 | (3S)-3,6-diaminohexanoate | Acetoanaerobium sticklandii | - |
(3S,5S)-3,5-diaminohexanoate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.4.3.3 | Acetoanaerobium sticklandii | E3PRJ5 AND E3PRJ4 | alpha- and beta-subunits | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
5.4.3.3 | (3S)-3,6-diaminohexanoate = (3S,5S)-3,5-diaminohexanoate | reaction mechanism, detailed overview | Acetoanaerobium sticklandii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.4.3.3 | (3S)-3,6-diaminohexanoate | - |
Acetoanaerobium sticklandii | (3S,5S)-3,5-diaminohexanoate | - |
r | |
5.4.3.3 | D-lysine | - |
Acetoanaerobium sticklandii | 2,5-diaminohexanoate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.4.3.3 | 5,6-LAM | - |
Acetoanaerobium sticklandii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.4.3.3 | 37 | - |
assay at | Acetoanaerobium sticklandii |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
5.4.3.3 | 1 | - |
D-Lysine | with pyridoxal 5'-phosphate N-oxide as cofactor, pH 8.5, 37°C | Acetoanaerobium sticklandii | |
5.4.3.3 | 14.2 | - |
D-Lysine | with pyridoxal 5'-phosphate as cofactor, pH 8.5, 37°C | Acetoanaerobium sticklandii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.4.3.3 | 8.5 | - |
assay at | Acetoanaerobium sticklandii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
5.4.3.3 | 5'-deoxyadenosylcobalamin | - |
Acetoanaerobium sticklandii | |
5.4.3.3 | additional information | lysine 5,6-aminomutase is a 5'-deoxyadenosylcobalamin and pyridoxal 5'-phosphate co-dependent radical enzyme, formation of internal aldimine with pyridoxal 5'-phosphate and pyridoxal 5'-phosphate-N-oxide | Acetoanaerobium sticklandii | |
5.4.3.3 | additional information | lysine 5,6-aminomutase shows flexibility toward cofactor analogue pyridoxal 5'-phosphate-N-oxide, which can act as a functional cofactor for enzyme 5,6-LAM, kinetics and structure-function analysis, overview | Acetoanaerobium sticklandii | |
5.4.3.3 | pyridoxal 5'-phosphate | - |
Acetoanaerobium sticklandii |