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Literature summary extracted from

  • Han, Z.; Sakai, N.; Boettger, L.H.; Klinke, S.; Hauber, J.; Trautwein, A.X.; Hilgenfeld, R.
    Crystal structure of the peroxo-diiron(III) intermediate of deoxyhypusine hydroxylase, an oxygenase involved in hypusination (2015), Structure, 23, 882-892 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.99.29 expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.29 in complex with glycerol, hanging drop vapor diffusion method, using 30% polyethylene glycol 6000 (w/v), 400 mM guanidinium hydrochloride, 100 mM Bis-Tris propane (pH 7.2-7.4). Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.99.29 Iron non-heme diiron enzyme Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.99.29 [eIF5A]-deoxyhypusine + AH2 + O2 Homo sapiens
-
 [eIF5A]-hypusine + A + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.29 Homo sapiens Q9BU89
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.99.29 GST column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.29 [eIF5A]-deoxyhypusine + AH2 + O2
-
 Homo sapiens [eIF5A]-hypusine + A + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.29 deoxyhypusine hydroxylase
-
 Homo sapiens
1.14.99.29 DOHH
-
 Homo sapiens