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Literature summary extracted from

  • Cao, S.; Li, H.; Yao, X.; Li, L.; Jiang, L.; Zhang, Q.; Zhang, J.; Liu, D.; Lu, H.
    Enzymatic characterization of two acetyl-CoA synthetase genes from Populus trichocarpa (2016), SpringerPlus, 5, 818 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.1 expressed in Escherichia coli BL21 cells Populus trichocarpa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.1 0.25
-
 Sodium acetate isoform ACS1, at pH 7.8 and 25°C Populus trichocarpa
6.2.1.1 0.72
-
 Sodium acetate isoform ACS2, at pH 7.8 and 25°C Populus trichocarpa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.1 ATP + acetate + CoA Populus trichocarpa
-
 AMP + diphosphate + acetyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.1 Populus trichocarpa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.1 Ni-NTA column chromatography Populus trichocarpa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.1 leaf highest expression Populus trichocarpa
-
6.2.1.1 phloem
-
 Populus trichocarpa
-
6.2.1.1 root
-
 Populus trichocarpa
-
6.2.1.1 xylem
-
 Populus trichocarpa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.1 ATP + acetate + CoA
-
 Populus trichocarpa AMP + diphosphate + acetyl-CoA
-
 ?
6.2.1.1 ATP + sodium acetate + CoA
-
 Populus trichocarpa AMP + acetyl-CoA + sodium diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.1 ? x * 50000, isoform ACS2, SDS-PAGE Populus trichocarpa
6.2.1.1 ? x * 60000, isoform ACS1, SDS-PAGE Populus trichocarpa

Synonyms

EC Number Synonyms Comment Organism
6.2.1.1 Acetyl-CoA synthase
-
 Populus trichocarpa
6.2.1.1 ACS1 isoform Populus trichocarpa
6.2.1.1 ACS2 isoform Populus trichocarpa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.1 35
-
-
 Populus trichocarpa

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.1 15 55 the enzyme activity is more than 90% of maximum over a broad temperature range between 25 and 40°C, and both recombinant isoform proteins retain more than 60% of their maximum enzymatic activity at temperatures between 15 and 45°C. Isoform ACS1 enzyme activity decreases to 12% of its maximum at 55°C Populus trichocarpa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.1 7.5
-
 isoform ACS1 Populus trichocarpa
6.2.1.1 8
-
 isoform ACS2 Populus trichocarpa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.1 5 9 isoform ACS1 shows high levels of activity in a pH range of 7.0-8.0. Isoform ACS2 shows high levels of activity in a pH range of 7.5-8.5. Little activity is detected for either isoform ACS1 or ACS2 at pH levels below 5.0 or above 9.0 Populus trichocarpa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.1 ATP
-
 Populus trichocarpa

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.1 Populus trichocarpa isoform ACS2, isoelectric focusing
-
 8.7
6.2.1.1 Populus trichocarpa isoform ACS1, isoelectric focusing
-
 8.8