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Literature summary extracted from

  • Li, J.; Xu, X.; Shi, P.; Liu, B.; Zhang, Y.; Zhang, W.
    Overexpression and characterization of a novel endo-beta-1,3(4)-glucanase from thermophilic fungus Humicola insolens Y1 (2017), Protein Expr. Purif., 138, 63-68 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.6 expression in Escherichia coli Humicola insolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.6 2-mercaptoethanol 1 mM, 93% residual activity Humicola insolens
3.2.1.6 Ag+ 1 mM, no residual activity Humicola insolens
3.2.1.6 Ca2+ 1 mM, 83% residual activity Humicola insolens
3.2.1.6 EDTA 1 mM, 91% residual activity Humicola insolens
3.2.1.6 Mn2+ 1 mM, 66% residual activity Humicola insolens
3.2.1.6 SDS 1 mM, 75% residual activity Humicola insolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
 Barley beta-glucan Km value 0.91 mg/ml, pH 5.5, 55°C Humicola insolens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Humicola insolens A0A0U2RV57
-
-
3.2.1.6 Humicola insolens Y1 A0A0U2RV57
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.6 693
-
 substrate barley beta-glucan, pH 5.5, 556°C Humicola insolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 barley beta-glucan + H2O
-
 Humicola insolens ?
-
 ?
3.2.1.6 barley beta-glucan + H2O
-
 Humicola insolens Y1 ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Humicola insolens ?
-
 ?
3.2.1.6 laminarin + H2O
-
 Humicola insolens Y1 ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Humicola insolens ?
-
 ?
3.2.1.6 lichenan + H2O
-
 Humicola insolens Y1 ?
-
 ?
3.2.1.6 additional information no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper Humicola insolens ?
-
 ?
3.2.1.6 additional information no substrates: carboxymethylcellulose, birchwood xylan, avicel and filter paper Humicola insolens Y1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.6 ? x * 32400, calculated Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 55
-
-
 Humicola insolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 50
-
 stable up to Humicola insolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5.5
-
-
 Humicola insolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.6 5.5 9
-
 Humicola insolens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.6 Humicola insolens calculated
-
 6