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Literature summary extracted from

  • Dotsenko, A.; Gusakov, A.; Rozhkova, A.; Sinitsyna, O.; Nemashkalov, V.; Sinitsyn, A.
    Effect of N-linked glycosylation on the activity and other properties of recombinant endoglucanase IIa (Cel5A) from Penicillium verruculosum (2016), Protein Eng. Des. Sel., 29, 495-501 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Penicillium canescens Talaromyces verruculosus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 N194A mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability. Slight shift of pH optimum 4.5 for wild-type to 5.0 and 32-35% increase in the specific activity against carboxymethylcellulose and barley beta-glucan Talaromyces verruculosus
3.2.1.4 N19A mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability but 26% decrease in the specific activity against carboxymethylcellulose and 12% against barley beta-glucan Talaromyces verruculosus
3.2.1.4 N42A mutation in potential N-glycosylation site, no notable effect on the enzyme thermostability. Slight shift of pH optimum 4.5 for wild-type to 5.0 and 12-13% increase in the specific activity against carboxymethylcellulose and barley beta-glucan Talaromyces verruculosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 additional information
-
 Barley beta-glucan Km value 20 mg/ml for wild-type, 13 mg/ml for mutant N19A, 21 mg/ml for mutant N42A, 22 mg/ml for mutant N194A, respectively, pH 5.0, 40°C Talaromyces verruculosus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Talaromyces verruculosus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 42
-
 mutant N19A, substrate carboxymethylcellulose, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 53
-
 mutant N19A, substrate barley beta-glucan, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 57
-
 wild-type, substrate carboxymethylcellulose, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 60
-
 wild-type, substrate barley beta-glucan, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 64
-
 mutant N42A, substrate carboxymethylcellulose, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 68
-
 mutant N42A, substrate barley beta-glucan, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 75
-
 mutant N194A, substrate carboxymethylcellulose, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 81
-
 mutant N194A, substrate barley beta-glucan, pH 5.0, 40°C Talaromyces verruculosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley beta-glucan + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O
-
 Talaromyces verruculosus ?
-
 ?
3.2.1.4 milled aspen wood + H2O
-
 Talaromyces verruculosus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel5A
-
 Talaromyces verruculosus
3.2.1.4 endoglucanase IIa
-
 Talaromyces verruculosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 127
-
 Barley beta-glucan mutant N19A, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 165
-
 Barley beta-glucan wild-type, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 227
-
 Barley beta-glucan mutant N42A, pH 5.0, 40°C Talaromyces verruculosus
3.2.1.4 275
-
 Barley beta-glucan mutant N194A, pH 5.0, 40°C Talaromyces verruculosus