BRENDA - Enzyme Database

Characterization of a novel CYP51 from Rhodococcus triatomae and its NADH-ferredoxin reductase-coupled application in lanosterol 14alpha-demethylation

Ke, X.; Ding, G.; Ma, B.; Liu, Z.; Zhang, J.; Zheng, Y.; Process Biochem. 62, 59-68 (2017)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.15.36
expression in Escherichia coli
Rhodococcus triatomae
Crystallization (Commentary)
EC Number
Crystallization
Organism
1.14.15.36
homoology modeling of structure
Rhodococcus triatomae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.3
0.0344
-
NADH
pH and temperature not specified in the publication
Acinetobacter sp.
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acinetobacter sp.
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Nocardia farcinica
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.15.36
Rhodococcus triatomae
M2UZT3
-
-
1.14.15.36
Rhodococcus triatomae BKS 15-14
M2UZT3
-
-
1.18.1.3
Acinetobacter sp.
-
-
-
1.18.1.3
Nocardia farcinica
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.36
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.36
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.36
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
746345
Rhodococcus triatomae
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
1.14.15.36
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
746345
Rhodococcus triatomae BKS 15-14
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
1.14.15.36
additional information
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
746345
Rhodococcus triatomae
?
-
-
-
-
1.14.15.36
additional information
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
746345
Rhodococcus triatomae BKS 15-14
?
-
-
-
-
1.14.15.36
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
746345
Acinetobacter sp.
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
746345
Nocardia farcinica
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.15.36
?
x * 54000, SDS-PAGE
Rhodococcus triatomae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
NADH
-
Acinetobacter sp.
1.18.1.3
NADH
-
Nocardia farcinica
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.15.36
expression in Escherichia coli
Rhodococcus triatomae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.18.1.3
NADH
-
Acinetobacter sp.
1.18.1.3
NADH
-
Nocardia farcinica
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.14.15.36
homoology modeling of structure
Rhodococcus triatomae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.18.1.3
0.0344
-
NADH
pH and temperature not specified in the publication
Acinetobacter sp.
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Acinetobacter sp.
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
Nocardia farcinica
-
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.15.36
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.36
hydroxylanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
oxolanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
-
-
?
1.14.15.36
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
lanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
hydroxylanosterol + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
746345
Rhodococcus triatomae
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
1.14.15.36
lanosterol + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2
-
746345
Rhodococcus triatomae BKS 15-14
4,4-dimethylzymosterol + formate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O
-
-
-
?
1.14.15.36
additional information
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
746345
Rhodococcus triatomae
?
-
-
-
-
1.14.15.36
additional information
exogenous electron donor systems, Fdx-FdR i.e. Acinetobacter sp. OC4 ferredoxin and ferredoxin reductase, Fld-FdR2 i.e. Escherichia coli flavodoxin and flavodoxin reductase, and Nocardia farcinica iron-sulfur containing NADPH-P450 reductase NfFdR can be used. Fdx-FdR is the most efficient electron donor
746345
Rhodococcus triatomae BKS 15-14
?
-
-
-
-
1.14.15.36
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.15.36
oxolanosterol + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2
-
746345
Rhodococcus triatomae BKS 15-14
4,4-dimethylzymosterol + formate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
746345
Acinetobacter sp.
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
1.18.1.3
oxidized [2Fe-2S] ferredoxin + NADH
-
746345
Nocardia farcinica
reduced [2Fe-2S] ferredoxin + NAD+ + H+
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.15.36
?
x * 54000, SDS-PAGE
Rhodococcus triatomae