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Literature summary extracted from

  • Zhang, Y.; Lee, C.; Wehner, N.; Imdahl, F.; Svetlana, V.; Weiste, C.; Droege-Laser, W.; Deeken, R.
    Regulation of oncogene expression in T-DNA-transformed host plant cells (2015), PLoS Pathog., 11, 1-27 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.3 gene IaaM, the gene is constitutively expressed in plant host Arabidopsis thaliana via Ti plasmid-encoded T-DNA insertion in tranfected cells, and no transcription factor further activates its promoter IGR1b, that contains cis-regulatory binding elements. Genetic organization, overview. Transcription factor ARR1 can regulate transcription of the IaaH or IaaM genes. Promoter IGR1 drives expression of IaaH and IaaM and contains only one Wbox and AuxRE sequence motif, and this is more closely localized upstream of the IaaM than that of the IaaH TATA box Agrobacterium tumefaciens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.3 L-tryptophan + O2 Agrobacterium tumefaciens
-
 (indol-3-yl)acetamide + CO2 + H2O
-
 ?
1.13.12.3 L-tryptophan + O2 Agrobacterium tumefaciens C58
-
 (indol-3-yl)acetamide + CO2 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.3 Agrobacterium tumefaciens Q9R717
-
-
1.13.12.3 Agrobacterium tumefaciens C58 Q9R717
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.3 L-tryptophan + O2
-
 Agrobacterium tumefaciens (indol-3-yl)acetamide + CO2 + H2O
-
 ?
1.13.12.3 L-tryptophan + O2
-
 Agrobacterium tumefaciens C58 (indol-3-yl)acetamide + CO2 + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.3 IaaM
-
 Agrobacterium tumefaciens
1.13.12.3 tryptophan monooxygenase
-
 Agrobacterium tumefaciens

Expression

EC Number Organism Comment Expression
1.13.12.3 Agrobacterium tumefaciens gene IaaM is constitutively expressed and no transcription factor further activates its promoter additional information

General Information

EC Number General Information Comment Organism
1.13.12.3 metabolism the enzyme is involved in the biosynthesis of auxin Agrobacterium tumefaciens
1.13.12.3 physiological function Crown gall development requires the expression of agrobacterial genes in the plant host, e.g. Arabidopsis thaliana. These genes are transferred by the T-DNA of the plant pathogen Agrobacterium tumefaciens and include the oncogenes IaaH, IaaM and Ipt, which, according to the tumor-inducing principle, are essential for crown gall development. Virulent Agrobacterium tumefaciens strains integrate their T-DNA into the plant genome where the encoded agrobacterial oncogenes are expressed and cause crown gall disease. Essential for crown gall development are IaaH (indole-3-acetamide hydrolase), IaaM (tryptophan monooxygenase) and Ipt (isopentenyl transferase), which encode enzymes for the biosynthesis of auxin (IaaH, IaaM) and cytokinin (Ipt). The oncogenes are involved in auxin and cytokinin production. The intergenic regions (IGRs) between the coding sequences (CDS) of the three oncogenes function as promoters in plant cells, but gene IaaM is constitutively expressed and no transcription factor further activates its promoter. With increasing auxin levels triggered by ubiquitous expression of IaaH and IaaM, ARF5 is activated and interacts with WRKY40 to potentiate Ipt expression and balance cytokinin and auxin levels for further cell proliferation. Transcriptional regulation of the Agrobacterium tumefaciens genes IaaH, IaaM and Ipt in the host plant, detailed overview Agrobacterium tumefaciens