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Literature summary extracted from

  • Jang, M.K.; Kim, J.H.; Jung, M.H.
    Histone H3K9 demethylase JMJD2B activates adipogenesis by regulating H3K9 methylation on PPARgamma and C/EBPalpha during adipogenesis (2017), PLoS ONE, 12, e0168185 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.66 gene KDM4B, quantitative real-time PCR enzyme expression analysis Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.14.11.66 additional information enzyme knockout by expression of siRNA targeting JMJD2B in 3T3-L1 preadipocytes Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.66 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.66 Fe2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.66 histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.66 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2 Homo sapiens
-
 histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.66 Homo sapiens O94953
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.11.66 3T3-L1 cell
-
 Homo sapiens
-
1.14.11.66 preadipocyte
-
 Homo sapiens
-
1.14.11.66 white adipose tissue
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.66 histone H3 N6,N6,N6-trimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6,N6-dimethyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.66 histone H3 N6,N6-dimethyl-L-lysine9 + 2-oxoglutarate + O2
-
 Homo sapiens histone H3 N6-methyl-L-lysine9 + succinate + formaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.66 histone H3K9 demethylase
-
 Homo sapiens
1.14.11.66 JMJD2B
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
1.14.11.66 evolution the enzyme JMJD2B belongs to the jumonji C (JmjC) domain-containing, iron-dependent dioxygenases (JMJC demethylases) Homo sapiens
1.14.11.66 malfunction JMJD2B knockdown using siRNA in 3T3-L1 preadipocytes represses the expression of PPARgamma and C/EBPalpha, resulting in inhibition of adipogenesis. This is accompanied by increased enrichment of H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha. In contrast, overexpression of JMJD2B increases the expression of PPARgamma and C/EBPalpha, which is accompanied by decreased enrichment of H3K9me3/me2 on the promoter and activated adipogenesis Homo sapiens
1.14.11.66 metabolism the methylation of lysine residues in histones is a main epigenetic modification in the regulation of eukaryotic gene expression. While methylation of histone H3 at the lysine 4 (H3K4) and 36 (H3K36) residues primarily associates with active transcription, methylation of histone H3 at the lysine 9 (H3K9) and 27 (H3K27) residues and histone H4 at lysine 20 (H4K20) associates with gene repression. Histone methylation by histone methyltransferase is antagonized by histone demethylases, which are divided into two classes: amine oxidases (LSD demethylases), and jumonji C (JmjC) domain-containing, iron-dependent dioxygenases (JMJC demethylases). Histone H3K9 demethylase JMJD2B decreases H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha, which in turn stimulated the expression of PPARgamma and C/EBPalpha. JMJD2B-mediated reduction of H3K9me3 increases H3K4me3 level on PPARgamma and C/EBPalpha Homo sapiens
1.14.11.66 physiological function histone H3K9 demethylase JMJD2B activates adipogenesis by regulating H3K9 methylation on PPARgamma and C/EBPalpha during adipogenesis in 3T3-L1 preadipocytes, overview. JMJD2B decreases H3K9me3/me2 on the promoter of PPARgamma and C/EBPalpha, which in turn stimulated the expression of PPARgamma and C/EBPalpha Homo sapiens