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Literature summary extracted from

  • Niu, C.; Zhu, L.; Xu, X.; Li, Q.
    Rational design of disulfide bonds increases thermostability of a mesophilic 1,3-1,4-beta-glucanase from Bacillus terquilensis (2016), PLoS ONE, 11, e0154036 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.73 expressed in Escherichia coli BL21(DE3) cells Bacillus tequilensis
3.2.1.73 expression in Escherichia coli Bacillus tequilensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.73 molecular dynamic simulation and and optimization of residue sites in the calcium region to increase the thermostability Bacillus tequilensis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.73 E46P/S43E/H205P/S40E 64% increase in specific activity, 20°C increase in optimal enzymatic temperature and a 13.8 °C rise in protein melting temperature compared to wild-type Bacillus tequilensis
3.2.1.73 N31C/T187C the mutations significantly enhance the protein thermostability Bacillus tequilensis
3.2.1.73 N31C/T187C/P102C/N125C introduction of disulfide pairs N31C-T187C and P102C-N125C, 48.3% increase in half-life value at 60°C and a 4.1°C rise in melting temperature, catalytic properties are similar to wild-type with a decrease in optimal pH Bacillus tequilensis
3.2.1.73 N31C/T187C/P102C/N125C the mutant with wild type activity shows a 48.3% increase in half-life value at 60°C and a 4.1°C rise in melting temperature compared to the wild type enzyme Bacillus tequilensis
3.2.1.73 P102C/N125C the mutations significantly enhance the protein thermostability Bacillus tequilensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.73 0.271
-
 azo blue barley beta-glucan mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 0.2773
-
 Barley beta-glucan mutant N31C/T187C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 0.2775
-
 Barley beta-glucan mutant N31C/T187C/P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 0.2777
-
 Barley beta-glucan mutant P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 0.2778
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 0.278
-
 azo blue barley beta-glucan mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 0.278
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 0.297
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.73 Bacillus tequilensis
-
-
-
3.2.1.73 Bacillus tequilensis CGX 5-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.73 Ni-NTA column chromatography Bacillus tequilensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.73 40 45 mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 2490
-
 wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 3936
-
 wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 4094
-
 mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C Bacillus tequilensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.73 azo blue barley beta-glucan + H2O
-
 Bacillus tequilensis ?
-
 ?
3.2.1.73 azo blue barley beta-glucan + H2O
-
 Bacillus tequilensis CGX 5-1 ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus tequilensis ?
-
 ?
3.2.1.73 barley beta-glucan + H2O
-
 Bacillus tequilensis CGX 5-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.73 1,3-1,4-beta-D-glucan 4-glucanohydrolase
-
 Bacillus tequilensis
3.2.1.73 1,3-1,4-beta-glucanase
-
 Bacillus tequilensis
3.2.1.73 BglT
-
 Bacillus tequilensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.73 45
-
 wild-type Bacillus tequilensis
3.2.1.73 60
-
-
 Bacillus tequilensis
3.2.1.73 65
-
 mutant E46P/S43E/H205P/S40E Bacillus tequilensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.73 50.8
-
 wild-type, melting temperature Bacillus tequilensis
3.2.1.73 54.9
-
 mutant N31C/T187C/P102C/N125C, melting temperature Bacillus tequilensis
3.2.1.73 60
-
 the melting temperature is 50.8°C. The half-life of the enzyme at 60°C is 59 min. The enzyme retains more than 60% of its activity at up to 75°C and is inactivated at 80°C after 10 min of incubation Bacillus tequilensis
3.2.1.73 62
-
 melting temperature, wild-type Bacillus tequilensis
3.2.1.73 70
-
 1 h, 60% residual activity, mutant E46P/S43E/H205P/S40E Bacillus tequilensis
3.2.1.73 76.5
-
 melting temperature, mutant E46P/S43E/H205P/S40E Bacillus tequilensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.73 137
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 183.6
-
 Barley beta-glucan mutant P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 184.9
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 185
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 186
-
 azo blue barley beta-glucan mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 186.3
-
 Barley beta-glucan mutant N31C/T187C/P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 187
-
 azo blue barley beta-glucan mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 187.6
-
 Barley beta-glucan mutant N31C/T187C, at pH 6.5 and 40°C Bacillus tequilensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.73 6
-
 mutant N31C/T187C/P102C/N125C Bacillus tequilensis
3.2.1.73 6.5
-
-
 Bacillus tequilensis
3.2.1.73 6.5
-
 wild-type Bacillus tequilensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.73 5.5 8 more than 75% activity between pH 5.5 and 8.0 Bacillus tequilensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.73 5.5 7.5 the enzyme retains about 60% activity at pH 5.5 and 90% at pH 7.5 after 10 min of incubation Bacillus tequilensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.73 462
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 660.4
-
 Barley beta-glucan mutant P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 665
-
 azo blue barley beta-glucan wild-type, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 665.1
-
 Barley beta-glucan wild type enzyme, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 672
-
 azo blue barley beta-glucan mutant N31C/T187C/P102C/N125C, pH 6.5, 40°C Bacillus tequilensis
3.2.1.73 672.6
-
 Barley beta-glucan mutant N31C/T187C/P102C/N125C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 677.3
-
 Barley beta-glucan mutant N31C/T187C, at pH 6.5 and 40°C Bacillus tequilensis
3.2.1.73 691
-
 azo blue barley beta-glucan mutant E46P/S43E/H205P/S40E, pH 6.5, 40°C Bacillus tequilensis