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Literature summary extracted from

  • Zhu, Y.; Peng, Q.Z.; Li, K.G.; Xie, D.Y.
    Molecular cloning and functional characterization of the anthocyanidin reductase gene from Vitis bellula (2014), Planta, 240, 381-398 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.77 gene ANR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-Trx-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana ban mutant Vitis bellula

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.77 additional information generation of an ANR T-DNA insertion that lack flavan-3-ols and proanthocyanidins Vitis bellula

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.77 NaCl purified recombinant VbANR almost loses its catalytic activity at 500 mM Vitis bellula

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.77 additional information
-
 additional information kinetics of Trx-VbANR with NADPH and NADH, overview Vitis bellula
1.3.1.77 0.1308
-
 delphinidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 0.1345
-
 pelargonidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 0.1757
-
 cyanidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 0.1929
-
 NADPH pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 0.8169
-
 NADH pH 4.0, 40°C, recombinant enzyme Vitis bellula

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.77 cyanidin + 2 NADPH + H+ Vitis bellula
-
 (2R,3R)-epicatechin + 2 NADP+ ? ?
1.3.1.77 delphinidin + 2 NADPH + H+ Vitis bellula
-
 (2R,3R)-epigallocatechin + 2 NADP+ ? ?
1.3.1.77 pelargonidin + 2 NADPH + H+ Vitis bellula
-
 (2R,3R)-epiafzelechin + 2 NADP+ ? ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.77 Vitis bellula H9TZS7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.77 recombinant His-Trx-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Vitis bellula

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.77 leaf
-
 Vitis bellula
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.77 anthocyanidin + 2 NADPH + H+
-
 Vitis bellula 2,3-trans-(2S,3R)-flavan-3-ol + 2 NADP+ ? ?
1.3.1.77 cyanidin + 2 NADH + H+
-
 Vitis bellula (2R,3R)-epicatechin + 2 NAD+ ? ?
1.3.1.77 cyanidin + 2 NADPH + H+
-
 Vitis bellula (2R,3R)-epicatechin + 2 NADP+ ? ?
1.3.1.77 delphinidin + 2 NADH + H+
-
 Vitis bellula (2R,3R)-epigallocatechin + 2 NAD+ ? ?
1.3.1.77 delphinidin + 2 NADPH + H+
-
 Vitis bellula (2R,3R)-epigallocatechin + 2 NADP+ ? ?
1.3.1.77 additional information the recombinant protein in vitro converts anthocyanidins to 2R,3R-cis-flavan-3-ols and 2S,3R-trans-flavan-3-ols, two stereo configurations at the C2 and C3 positions, such as (-)-epicatechin and (-)-catechin. flavan-3-ols produced from the catalysis of Trx-VbANR result from the reduction activity of VbANR but not Trx. Stereochemistry of the enzyme reaction, overview Vitis bellula ?
-
 ?
1.3.1.77 pelargonidin + 2 NADH + H+
-
 Vitis bellula (2R,3R)-epiafzelechin + 2 NAD+ ? ?
1.3.1.77 pelargonidin + 2 NADPH + H+
-
 Vitis bellula (2R,3R)-epiafzelechin + 2 NADP+ ? ?

Subunits

EC Number Subunits Comment Organism
1.3.1.77 ? x * 56000, recombinant His-Trx-tagged enzyme, SDS-PAGE Vitis bellula

Synonyms

EC Number Synonyms Comment Organism
1.3.1.77 ANR
-
 Vitis bellula
1.3.1.77 anthocyanidin reductase
-
 Vitis bellula
1.3.1.77 VbANR
-
 Vitis bellula

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.77 40
-
 recombinant enzyme Vitis bellula

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.77 30 50 activity profile, overview Vitis bellula

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.77 1.665
-
 cyanidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 2
-
 pelargonidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 2.012
-
 delphinidin pH 4.0, 40°C, recombinant enzyme Vitis bellula

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.77 4
-
 recombinant enzyme Vitis bellula

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.77 3 8 activity range, profile overview Vitis bellula

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.77 additional information enzyme VbANR uses both NADPH and NADH but prefers to employ NADPH Vitis bellula
1.3.1.77 NADH
-
 Vitis bellula
1.3.1.77 NADPH
-
 Vitis bellula

General Information

EC Number General Information Comment Organism
1.3.1.77 additional information the putative enzymatic activity triad is composed of Ser136, Tyr173, and Lys177 Vitis bellula
1.3.1.77 physiological function anthocyanidin reductase (ANR) is an NADPH-/NADH-dependent enzyme that transfers two hydrides to anthocyanidins to produce three types of isomeric flavan-3-ols Vitis bellula

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.1.77 9.48
-
 cyanidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 14.87
-
 pelargonidin pH 4.0, 40°C, recombinant enzyme Vitis bellula
1.3.1.77 15.38
-
 delphinidin pH 4.0, 40°C, recombinant enzyme Vitis bellula