BRENDA - Enzyme Database

Overexpression of a tea flavanone 3-hydroxylase gene confers tolerance to salt stress and Alternaria solani in transgenic tobacco

Mahajan, M.; Yadav, S.K.; Plant Mol. Biol. 85, 551-573 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt
Camellia sinensis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Camellia sinensis
-
dihydrokaempferol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydrokaempferol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Camellia sinensis
Q6DV45
from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-
1.14.11.9
Camellia sinensis UPASI-10
Q6DV45
from the tea garden at the CSIR-Institute of Himalayan Bioresource Technology, Palampur
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydrokaempferol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
CsF3H
-
Camellia sinensis
1.14.11.9
flavanone 3-hydroxylase
-
Camellia sinensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
overexpression of the Camellia sinensis flavanone 3-hydroxylase gene in leaves of transgenic Nicotiana tabacum cv. Xanthi confers tolerance to salt stress and pathogenic fungus Alternaria solani, transformation via Agrobacterium tumefaciens strain LBA4404 containing pCAMBIA-F3H. A decrease in pectin methyl esterase (PME) activity and increase in pectin methyl esterification is also observed in CsF3H transgenic tobacco plants. Transcript expression analysis, and analysis of trangenic PME activity in flavonoid (epigallocatechin)-exposed and -unexposed seedlings, and in seedlings exposed to salt
Camellia sinensis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydroquercetin + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Camellia sinensis
-
dihydrokaempferol + succinate + CO2
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
Camellia sinensis UPASI-10
-
dihydrokaempferol + succinate + CO2
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.11.9
leaf
-
Camellia sinensis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis
dihydrokaempferol + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
746091
Camellia sinensis UPASI-10
dihydrokaempferol + succinate + CO2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
metabolism
flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis
Camellia sinensis
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
metabolism
flavanone 3-hydroxylase (F3H) of the flavonoid pathway catalyzes the stereospecific hydroxylation of (2S)-naringenin and (2S)-eriodictyol to form (2R,3R)-dihydrokaempferol and (2R,3R)-dihydroquercetin, respectively. These dihydroflavonols serve as intermediates for the biosynthesis of flavan-3-ols. Enzyme F3H plays a pivotal role in regulation of biosynthesis of flavan-3-ols in Camellia sinensis
Camellia sinensis