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Literature summary extracted from

  • Noe, W.; Mollenschott, C.; Berlin, J.
    Tryptophan decarboxylase from Catharanthus roseus cell suspension cultures purification, molecular and kinetic data of the homogenous protein (1984), Plant Mol. Biol., 3, 281-288 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.105 D-tryptophan non-competitive inhibitor Catharanthus roseus
4.1.1.105 additional information not inhibited by CO2 Catharanthus roseus
4.1.1.105 tryptamine competitive inhibitor Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.105 0.075
-
 L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus
4.1.1.105 1.3
-
 5-hydroxy-L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.105 115000
-
 gel filtration Catharanthus roseus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.105 L-tryptophan Catharanthus roseus
-
 tryptamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.105 Catharanthus roseus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.105 ammonium sulfate precipitation, DEAE-Sephacel column chromatography, hydroxylapatite column chromatography, AcA-34 column chromatography, TSK-4000 gel filtration, Mono Q column chromatography, and TSK-3000 gel filtration Catharanthus roseus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Storage Stability

EC Number Storage Stability Organism
4.1.1.105 0°C, purified enzyme at pH 7.5, 3 weeks, 90% loss of activity Catharanthus roseus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.105 5-hydroxy-L-tryptophan
-
 Catharanthus roseus 5-hydroxytryptamine + CO2
-
 ?
4.1.1.105 L-tryptophan
-
 Catharanthus roseus tryptamine + CO2
-
 ?
4.1.1.105 additional information L-phenylalanine, L-tyrosine and DOPA are not decarboxylated by the enzyme Catharanthus roseus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.105 homodimer 2 * 54000, SDS-PAGE Catharanthus roseus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.105 TDC
-
 Catharanthus roseus
4.1.1.105 Tryptophan decarboxylase
-
 Catharanthus roseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.105 3.33
-
 L-tryptophan at pH 7.5, temperature not specified in the publication Catharanthus roseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.105 8.5
-
-
 Catharanthus roseus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.105 pyridoxal 5'-phosphate
-
 Catharanthus roseus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.1.105 0.31
-
 tryptamine at pH 7.5, temperature not specified in the publication Catharanthus roseus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.105 Catharanthus roseus isoelectric focusing
-
 5.9