Literature summary extracted from

Zhang, L.; Li, H.; Gao, L.; Qi, Y.; Fu, W.; Li, X.; Zhou, X.; Gao, Q.; Gao, Z.; Jia, H.
Acyl-CoA oxidase 1 is involved in gamma-decalactone release from peach (Prunus persica) fruit (2017), Plant Cell Rep., 36, 829-842 .

Activating Compound

EC Number	Activating Compound	Comment	Organism	Structure
1.3.3.6	CoA	co-treatment with ACX1 and CoA significantly promoted the biosynthesis of gamma-decalactone compared to the control	Prunus persica
1.3.3.6	additional information	exogenous application of recombinant PpACX1 and C16-CoA substrate markedly promots gamma-decalactone biosynthesis, while C16-CoA substrate application alone does not promote its biosynthesis in mesocarp discs cultured in vitro	Prunus persica

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
1.3.3.6	gene ACX1, real-time quantitative PCR enzyme expression analysis in fruit from the different cultivars, recombinant expression of His-tagged enzyme in from vector pET32a in Escherichia coli	Prunus persica

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
1.3.3.6	Mg2+	no effect on enzyme activity up to 20 mM, inhibitory above, 40% inhibition at 30 mM	Prunus persica
1.3.3.6	Mn2+	has no significant effect on enzyme activity up to 10 mM	Prunus persica

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.3.3.6	additional information	-	additional information	Michaelis-Menten hyperbolic kinetic model	Prunus persica

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
1.3.3.6	acyl-CoA + O2	Prunus persica	-	trans-2,3-dehydroacyl-CoA + H2O2	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
1.3.3.6	Prunus persica	J7K6M3	five cvs., two soft melting peaches Hu Jing Mi Lu and Feng Hua Yu Lu with strong aroma, one hard melting peach Tai Gu Rou Tao with intermediate aroma, and two hard melting peaches Zhong Hua Shou Tao and Zhong Nectarine 16 with slight aroma	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
1.3.3.6	recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Prunus persica

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
1.3.3.6	fruit	real-time quantitative PCR enzyme expression analysis in fruit from the different cultivars	Prunus persica	-
1.3.3.6	mesocarp	isozyme PpACX1 is the most abundant PpACX protein in fully ripe mesocarp of cultivar Hu Jing Mi Lu	Prunus persica	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
1.3.3.6	acyl-CoA + O2	-	Prunus persica	trans-2,3-dehydroacyl-CoA + H2O2	-	?
1.3.3.6	additional information	PpACX1 exhibits activity with medium- to long-chain fatty acyl-CoAs, equally for C10-CoA to C16-CoA. Isozyme PpACX1 activity using C6-CoA and C10-CoA as substrates is relatively lower and both maintains constant during the reaction time, while a linear activity with C16-CoA substrate is observed	Prunus persica	?	-	?

Subunits

EC Number	Subunits	Comment	Organism
1.3.3.6	?	x * 94440, recombinant enzyme including the pET32a vector encoding a 20.10 kDa protein, MALDITOF/TOF secondary mass spectrometry, x * 74340, sequence calculation and SDS-PAGE	Prunus persica

Synonyms

EC Number	Synonyms	Comment	Organism
1.3.3.6	ACX	-	Prunus persica
1.3.3.6	acyl-CoA oxidase 1	-	Prunus persica
1.3.3.6	PpACX1	-	Prunus persica

Temperature Optimum [°C]

EC Number	Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
1.3.3.6	22	-	assay at room temperature	Prunus persica

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
1.3.3.6	7.6	-	assay at	Prunus persica

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
1.3.3.6	FAD	-	Prunus persica

General Information

EC Number	General Information	Comment	Organism
1.3.3.6	physiological function	acyl-CoA oxidase 1 is involved in gamma-decalactone release from Prunus persica fruits. gamma-Decalactone accumulation in peach mesocarp is highly correlated with ACX enzyme activity and natural PpACX1 content. Adding the purified recombinant PpACX1 induces gamma-decalactone biosynthesis in cultured mesocarp discs in vitro	Prunus persica

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Release 2023.1 (January 2023)