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Literature summary extracted from

  • Bhunia, R.K.; Chakraborty, A.; Kaur, R.; Maiti, M.K.; Sen, S.K.
    Enhancement of alpha-linolenic acid content in transgenic tobacco seeds by targeting a plastidial omega-3 fatty acid desaturase (fad7) gene of Sesamum indicum to ER (2016), Plant Cell Rep., 35, 213-226 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.19.35 nutrition potential of the engineered plastidial omega-3 desaturase from sesame to influence the profile of alpha-linolenic acid in transgenic tobacco plant by shifting the carbon flux from linoleic acid, the enzyme can be used in suitable genetic engineering strategy to increase the alpha-linolenic acid content in sesame and other vegetable oils Sesamum indicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.35 gene fad7, sequence comparisons, recombinant functional expression in Nicotiana tabacum cv. Jayanti seeds and leaves targeted to the endoplasmic reticulum, using the Agrobacterium tumefaciens strain EHA105 transfection method and the binary vector pCAMBIA1300 with CaMV35S promoter, quantitative real-time PCR expression analysis Sesamum indicum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.35 additional information the predicted N-terminal plastidial signal peptide of fad7 gene is replaced by an endoplasmic reticulum signal peptide and an endoplasmic reticulum retention signal is placed at the C-terminal, enhancement of alpha-linolenic acid content in transgenic tobacco seeds by targeting the plastidial omega-3 fatty acid desaturase (fad7) gene from Sesamum indicum to the endoplasmic reticulum Sesamum indicum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.19.35 chloroplast at the inner membrane of the chloroplast envelope, the gene possesses a chloroplast targeting signal sequence Sesamum indicum 9507
-
1.14.19.35 chloroplast envelope
-
 Sesamum indicum 9941
-
1.14.19.35 thylakoid membrane
-
 Sesamum indicum 42651
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Sesamum indicum
-
 alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ Sesamum indicum Var-9
-
 alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.35 Sesamum indicum P48620
-
-
1.14.19.35 Sesamum indicum Var-9 P48620
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.19.35 leaf young Sesamum indicum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Sesamum indicum alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.19.35 alpha-linoleoyl-[glycerolipid] + 2 reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+
-
 Sesamum indicum Var-9 alpha-linolenoyl-[glycerolipid] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O
-
 ?
1.14.19.35 additional information FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol Sesamum indicum ?
-
 ?
1.14.19.35 additional information FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol Sesamum indicum Var-9 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.35 FAD7
-
 Sesamum indicum
1.14.19.35 fatty acid desaturase-7
-
 Sesamum indicum
1.14.19.35 plastidial omega-3 fatty acid desaturase
-
 Sesamum indicum
1.14.19.35 Sefad7
-
 Sesamum indicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.35 Ferredoxin FAD7 utilizes ferredoxin as the intermediate electron donor to act upon fatty acids esterified to galactolipids, sulfolipid, and phosphatidylglycerol Sesamum indicum