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Literature summary extracted from

  • Gao, S.; Yu, H.N.; Xu, R.X.; Cheng, A.X.; Lou, H.X.
    Cloning and functional characterization of a 4-coumarate CoA ligase from liverwort Plagiochasma appendiculatum (2015), Phytochemistry, 111, 48-58 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.12 expressed in Escherichia coli BL21(DE3) cells Plagiochasma appendiculatum

Protein Variants

EC Number Protein Variants Comment Organism
6.2.1.12 A251S the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme Plagiochasma appendiculatum
6.2.1.12 A251S/M247Y the mutant enzyme shows a lower binding affinity and catalytic efficiency than the wild type enzyme Plagiochasma appendiculatum
6.2.1.12 M247Y the catalytic efficiency of, 4-coumaric, caffeic and dihydro-4-coumaric acids are higher in the mutant than in the wild type enzyme Plagiochasma appendiculatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.12 0.155
-
 4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.1887
-
 4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.1929
-
 caffeate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.2089
-
 caffeate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.253
-
 dihydro-4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.283
-
 4-coumarate mutant enzyme M247Y/A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.389
-
 4-coumarate mutant enzyme A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.4418
-
 dihydro-4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA Plagiochasma appendiculatum 4-coumarate is the most favorable substrate AMP + diphosphate + 4-coumaroyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.12 Plagiochasma appendiculatum A0A0D4C3L0
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.12 0.008 4 mutant enzyme M247Y/A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0093
-
 mutant enzyme A251S, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0135
-
 mutant enzyme A251S, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0152
-
 mutant enzyme M247Y/A251S, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0161
-
 mutant enzyme M247Y/A251S, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0204
-
 wild type enzyme, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0231
-
 mutant enzyme A251S, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0344
-
 mutant enzyme M247Y, with dihydro-4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0378
-
 wild type enzyme, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0409
-
 wild type enzyme, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0577
-
 mutant enzyme M247Y/A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.0706
-
 mutant enzyme A251S, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.1079
-
 mutant enzyme M247Y, with cinnamate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.1136
-
 wild type enzyme, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.1309
-
 mutant enzyme M247Y, with caffeate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.4561
-
 mutant enzyme M247Y, with 4-coumarate as substrate, at pH 7.5 and 30°C Plagiochasma appendiculatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.12 ATP + 4-coumarate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + 4-coumarate + CoA 4-coumarate is the most favorable substrate Plagiochasma appendiculatum AMP + diphosphate + 4-coumaroyl-CoA
-
 ?
6.2.1.12 ATP + caffeate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + caffeoyl-CoA
-
 ?
6.2.1.12 ATP + cinnamate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + cinnamoyl-CoA
-
 ?
6.2.1.12 ATP + dihydro-4-coumarate + CoA
-
 Plagiochasma appendiculatum AMP + diphosphate + dihydro-4-coumaroyl-CoA
-
 ?
6.2.1.12 additional information no activity with ferulate, 5-hydroxyferylate, and sinapate Plagiochasma appendiculatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.12 ? x * 50920, calculated from amino acid sequence Plagiochasma appendiculatum
6.2.1.12 ? x * 80000, thioredoxin-, His- and S-tagged enzyme, SDS-PAGE Plagiochasma appendiculatum

Synonyms

EC Number Synonyms Comment Organism
6.2.1.12 4-coumarate CoA ligase
-
 Plagiochasma appendiculatum
6.2.1.12 4-coumarate: coenzyme A ligase
-
 Plagiochasma appendiculatum
6.2.1.12 4CL1
-
 Plagiochasma appendiculatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.2.1.12 0.084
-
 caffeate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.084
-
 dihydro-4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.098
-
 dihydro-4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.123
-
 4-coumarate mutant enzyme M247Y/A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.216
-
 4-coumarate wild type enzyme, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.217
-
 caffeate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.221
-
 4-coumarate mutant enzyme A251S, at pH 7.5 and 30°C Plagiochasma appendiculatum
6.2.1.12 0.782
-
 4-coumarate mutant enzyme M247Y, at pH 7.5 and 30°C Plagiochasma appendiculatum

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.12 ATP
-
 Plagiochasma appendiculatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
6.2.1.12 Plagiochasma appendiculatum calculated from amino acid sequence
-
 9.7

Expression

EC Number Organism Comment Expression
6.2.1.12 Plagiochasma appendiculatum enzyme gene transcription is induced when Plagiochasma appendiculatum thallus is treated with either salicylic acid or methyl jasmonate up