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Literature summary extracted from

  • Seo, D.; Asano, T.
    C-terminal residues of ferredoxin-NAD(P)+ reductase from Chlorobaculum tepidum are responsible for reaction dynamics in the hydride transfer and redox equilibria with NADP+/NADPH (2018), Photosynth. Res., 136, 275-290 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.18.1.2 additional information deletions of Tyr326-Glu360 decrease the hydride transfer rate and the amount of reduced enzyme increases at equilibrium relative to wild type. Deletions of Phe337-Glu360 and Ser338-Glu360 result in slight changes in the reaction kinetics and redox equilibrium compared to the wild type enzyme Chlorobaculum tepidum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.0142
-
 NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 91000
-
 gel filtration Chlorobaculum tepidum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+ + H+ Chlorobaculum tepidum
-
 oxidized ferredoxin + NADPH
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Chlorobaculum tepidum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 ferricyanide + NADPH
-
 Chlorobaculum tepidum ferrocyanide + NADP+ + H+
-
 r
1.18.1.2 reduced ferredoxin + NADP+ + H+
-
 Chlorobaculum tepidum oxidized ferredoxin + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 ? x * 42000, SDS-PAGE Chlorobaculum tepidum
1.18.1.2 ? x * 39190, MALDI-TOF mass spectrometry Chlorobaculum tepidum
1.18.1.2 ? x * 39240, calculated from amino acid sequence Chlorobaculum tepidum

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin-NAD(P)+ reductase
-
 Chlorobaculum tepidum
1.18.1.2 FNR
-
 Chlorobaculum tepidum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 213
-
 NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 NADP+
-
 Chlorobaculum tepidum
1.18.1.2 NADPH
-
 Chlorobaculum tepidum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 15000
-
 NADPH with 2 mM ferricyanide in 20 mM HEPES-NaOH buffer, at pH 7.0 and 25°C Chlorobaculum tepidum