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Literature summary extracted from

  • Eibinger, M.; Sattelkow, J.; Ganner, T.; Plank, H.; Nidetzky, B.
    Single-molecule study of oxidative enzymatic deconstruction of cellulose (2017), Nat. Commun., 8, 894 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.99.54 single-molecule study by atomic force microscopy Neurospora crassa
1.14.99.56 single-molecule study by atomic force microscopy Neurospora crassa

Organism

EC Number Organism UniProt Comment Textmining
1.14.99.54 Neurospora crassa
-
-
-
1.14.99.56 Neurospora crassa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.99.54 cellulose + reduced acceptor + O2
-
 Neurospora crassa ? + oxidized acceptor + H2O
-
 ?
1.14.99.56 cellulose + reduced acceptor + O2
-
 Neurospora crassa ? + oxidized acceptor + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.99.54 LPMO9C
-
 Neurospora crassa
1.14.99.54 LPMO9f
-
 Neurospora crassa
1.14.99.56 LPMO9C
-
 Neurospora crassa
1.14.99.56 LPMO9f
-
 Neurospora crassa

General Information

EC Number General Information Comment Organism
1.14.99.54 physiological function isoforms LPMO9C and LPMO9F bind to nanocrystalline cellulose with high preference for the very same substrate surfaces that are also used by Hypocrea jecorina processive cellulase CBH I to move along during hydrolytic cellulose degradation. The bound LPMOs are immobile during their adsorbed residence time on cellulose. Treatment with LPMO results in fibrillation of crystalline cellulose, enhances the cellulase adsorption more than 2fold and increases enzyme turnover on the cellulose surface, boosting the hydrolytic conversion Neurospora crassa
1.14.99.56 physiological function isoforms LPMO9C and LPMO9F bind to nanocrystalline cellulose with high preference for the very same substrate surfaces that are also used by Hypocrea jecorina processive cellulase CBH I to move along during hydrolytic cellulose degradation. The bound LPMOs are immobile during their adsorbed residence time on cellulose. Treatment with LPMO results in fibrillation of crystalline cellulose and more than 2fold enhances the cellulase adsorption. It also increased enzyme turnover on the cellulose surface, thus boosting the hydrolytic conversion Neurospora crassa