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Literature summary extracted from

  • Scheler, U.; Brandt, W.; Porzel, A.; Rothe, K.; Manzano, D.; Bozic, D.; Papaefthimiou, D.; Balcke, G.; Henning, A.; Lohse, S.; Marillonnet, S.; Kanellis, A.; Ferrer, A.; Tissier, A.
    Elucidation of the biosynthesis of carnosic acid and its reconstitution in yeast (2016), Nat. Commun., 7, 12942 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.61 expression in Nicotiana benthamiana leaf Salvia rosmarinus
1.14.14.61 expression in Nicotiana benthamiana leaf Salvia fruticosa
1.14.14.175 expression in Saccharomyces cerevisiae Salvia miltiorrhiza

Protein Variants

EC Number Protein Variants Comment Organism
1.14.14.175 D301E/N303S/V479F mutation leads to a second oxidation of product to give 11-hydroxyferruginol, i.e. reaction of EC 1.14.14.60 Salvia miltiorrhiza

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.60 Salvia fruticosa
-
-
-
1.14.14.60 Salvia rosmarinus
-
-
-
1.14.14.61 Salvia fruticosa
-
-
-
1.14.14.61 Salvia pomifera A0A0S1TPC7
-
-
1.14.14.61 Salvia rosmarinus
-
-
-
1.14.14.175 Salvia fruticosa
-
-
-
1.14.14.175 Salvia miltiorrhiza S4UX02
-
-
1.14.14.175 Salvia rosmarinus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.61 trichome glandular trichome Salvia rosmarinus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.60 ferruginol + [reduced NADPH-hemoprotein reductase] + O2 enzymes carry out not a single but two successive oxidations leading to 11-hydroxyferruginol from abieta-8,11,13-triene, i.e. reactions of EC 1.14.14.175 and 1.14.14.60 Salvia rosmarinus 11-hydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.60 ferruginol + [reduced NADPH-hemoprotein reductase] + O2 enzymes carry out not a single but two successive oxidations leading to 11-hydroxyferruginol from abieta-8,11,13-triene, i.e. reactions of EC 1.14.14.175 and 1.14.14.60 Salvia fruticosa 11-hydroxyferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.61 11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Salvia rosmarinus carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.61 11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Salvia pomifera carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.61 11-hydroxyferruginol + 3 [reduced NADPH-hemoprotein reductase] + 3 O2
-
 Salvia fruticosa carnosic acid + 3 [oxidized NADPH-hemoprotein reductase] + 4 H2O
-
 ?
1.14.14.61 additional information reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate Salvia rosmarinus ?
-
 ?
1.14.14.61 additional information reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, the latter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate Salvia pomifera ?
-
 ?
1.14.14.61 additional information reaction intermediates are 11,20-dohydroxyferruginol and carnosaldehyde, which itself spontaneously oxidizes to carnosol. The enzyme can also oxidize miltiradiene and ferruginol, thelatter leading to pisifieric acid, albeit with much lower efficiency, indicating 11-hydroxyferruginol is their preferred substrate Salvia fruticosa ?
-
 ?
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2
-
 Salvia miltiorrhiza ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 enzymes carry out not a single but two successive oxidations leading to 11-hydroxyferruginol from abieta-8,11,13-triene, i.e. reactions of EC 1.14.14.175 and 1.14.14.60 Salvia rosmarinus ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.175 abieta-8,11,13-triene + [reduced NADPH-hemoprotein reductase] + O2 enzymes carry out not a single but two successive oxidations leading to 11-hydroxyferruginol from abieta-8,11,13-triene, i.e. reactions of EC 1.14.14.175 and 1.14.14.60 Salvia fruticosa ferruginol + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.60 Cyp76AH22
-
 Salvia rosmarinus
1.14.14.60 Cyp76AH22
-
 Salvia fruticosa
1.14.14.60 Cyp76AH23
-
 Salvia rosmarinus
1.14.14.60 Cyp76AH23
-
 Salvia fruticosa
1.14.14.60 CYP76AH24
-
 Salvia rosmarinus
1.14.14.60 CYP76AH24
-
 Salvia fruticosa
1.14.14.61 CYP76AK6
-
 Salvia pomifera
1.14.14.61 CYP76AK7
-
 Salvia fruticosa
1.14.14.61 CYP76AK8
-
 Salvia rosmarinus
1.14.14.61 CYP76AK8
-
 Salvia fruticosa
1.14.14.175 CYP76AH1
-
 Salvia miltiorrhiza
1.14.14.175 Cyp76AH22
-
 Salvia rosmarinus
1.14.14.175 Cyp76AH22
-
 Salvia fruticosa
1.14.14.175 Cyp76AH23
-
 Salvia rosmarinus
1.14.14.175 Cyp76AH23
-
 Salvia fruticosa
1.14.14.175 CYP76AH24
-
 Salvia rosmarinus
1.14.14.175 CYP76AH24
-
 Salvia fruticosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.60 cytochrome P450
-
 Salvia rosmarinus
1.14.14.60 cytochrome P450
-
 Salvia fruticosa
1.14.14.175 cytochrome P450
-
 Salvia rosmarinus
1.14.14.175 cytochrome P450
-
 Salvia miltiorrhiza
1.14.14.175 cytochrome P450
-
 Salvia fruticosa