BRENDA - Enzyme Database

Heterologous expression of PA8FAD9 and functional characterization of a DELTA9-fatty acid desaturase from a cold-tolerant Pseudomonas sp. A8

Garba, L.; Ali, M.S.; Oslan, S.N.; Rahman, R.N.; Mol. Biotechnol. 58, 718-728 (2016)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.14.19.28
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein
Pseudomonas sp.
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A8(2016b)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.19.28
Pseudomonas sp.
A0A165DNG6
-
-
1.14.19.28
Pseudomonas sp. A8(2016b)
A0A165DNG6
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp. A8(2016b)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 45000, about, sequence calculation
Pseudomonas sp.
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Pseudomonas sp.
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.19.28
gene PA8FAD9, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli strain Transetta (DE3), ability of the enzyme to convert exogenous stearic acid to oleic acid incorporated into the recombinant Escherichia coli membrane phospholipids. GCMS analysis confirms significant changes in the pattern of palmitoleic and palmitic acid production in Escherichia coli cells expressing PA8FAD9 gene. At 20°C, the amount of palmitoleic increased from 10.5 to 21% suggesting that PA8FAD9 gene from Pseudomonas sp. A8 codes for an active DELTA9-fatty acid desaturase-like protein
Pseudomonas sp.
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.19.28
Ferredoxin
-
Pseudomonas sp.
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.19.28
membrane
-
Pseudomonas sp.
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp.
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
Pseudomonas sp. A8(2016b)
-
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp.
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
1.14.19.28
1-stearoyl-2-acyl-[glycerolipid] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
-
745749
Pseudomonas sp. A8(2016b)
1-oleoyl-2-acyl-[glycerolipid] + oxidized ferredoxin [iron-sulfur] cluster + H2O
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.14.19.28
?
x * 45000, about, sequence calculation
Pseudomonas sp.
General Information
EC Number
General Information
Commentary
Organism
1.14.19.28
physiological function
the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes
Pseudomonas sp.
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.19.28
physiological function
the D9-fatty acid desaturase initiates the synthesis of polyunsaturated fatty acids by introducing the first double bond at the C9 position of saturated fatty acid chains. These substrates can further be elongated by other classes of desaturase enzymes
Pseudomonas sp.