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Literature summary extracted from

  • Bullard, W.; Cliffe, L.; Wang, P.; Wang, Y.; Sabatini, R.
    Base J glucosyltransferase does not regulate the sequence specificity of J synthesis in trypanosomatid telomeric DNA (2015), Mol. Biochem. Parasitol., 204, 77-80 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.6 thymine + 2-oxoglutarate + O2 Leishmania major
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 5-hydroxymethyluracil + succinate + CO2
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.6 Leishmania major Q4QFY1
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-
1.14.11.6 Leishmania major Q4QHM7
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.6 thymine + 2-oxoglutarate + O2
-
 Leishmania major 5-hydroxymethyluracil + succinate + CO2
-
 ?
1.14.11.6 thymine + 2-oxoglutarate + O2 the telomeric repeat sequence GGGTTA is able to stimulate J synthesis, while mutated telomeric sequences (GGGTTT, GGGATT, or GGGAAA) are unable to support J synthesis Leishmania major 5-hydroxymethyluracil + succinate + CO2
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.6 JBP1
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 Leishmania major
1.14.11.6 JBP2
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 Leishmania major
1.14.11.6 thymidine hydroxylase
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 Leishmania major

General Information

EC Number General Information Comment Organism
1.14.11.6 physiological function telomeric DNA of Trypanosomatids possesses a modified thymine base, called base J or beta-D-glucopyranosyloxymethyluracil, that is synthesized in a two-step process. The base is hydroxylated by a thymidine hydroxylase forming hydroxymethyluracil (hmU), a glucose moiety is then attached by the J-associated glucosyltransferase (JGT). Both JBP1 and JBP2 stimulate de novo thymidine hydroxylation in vivo. DNA Jaylation is largely regulated by cis-acting sequences and is thus genetically encoded. The key regulatory step of J synthesis seems to be the first step catalyzed by JBP1 and JBP2. The specificity of base J localization is probably due to the JBP enzymes generating 5-hydroxymethyluracil at only specific sites throughout the genome Leishmania major