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Literature summary extracted from

  • Chen, H.; Xu, H.; Kweon, O.; Chen, S.; Cerniglia, C.
    Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis (2008), Microbiology, 154, 2659-2667 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.17 expression in Escherichia coli Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.17 W105A complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
1.7.1.17 W105F 31fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
1.7.1.17 W105G complete loss of both affinity for FMN and enzyme activity Enterococcus faecalis
1.7.1.17 W105H 8fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
1.7.1.17 W105Q 68fold decrease in vmax value, Km value similar to wild-type Enterococcus faecalis
1.7.1.17 W105Y 22% reduction in vmax value Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.17 0.0247
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105F, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.0251
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105H, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.0254
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid wild-type, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.0276
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105Y, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.0282
-
 4'-(dimethylamino)-azobenzene-2-carboxylic acid mutant W105Q, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.158
-
 NADH wild-type, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.166
-
 NADH mutant W105Y, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.169
-
 NADH mutant W105Q, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.171
-
 NADH mutant W105H, pH 7.1, 23°C Enterococcus faecalis
1.7.1.17 0.175
-
 NADH mutant W105F, pH 7.1, 23°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.17 Enterococcus faecalis Q831B2
-
-
1.7.1.17 Enterococcus faecalis ATCC 700802 Q831B2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?
1.7.1.17 4'-(dimethylamino)-azobenzene-2-carboxylic acid + 2 NADH + 2 H+ i.e. methyl red Enterococcus faecalis ATCC 700802 2-aminobenzoic acid + N,N-dimethyl-p-phenylenediamine + 2 NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.17 AzoA
-
 Enterococcus faecalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.17 FMN residues Trp105, Asn106, Leu107, Gly150, Gly151, Tyr153, Asn121 and Tyr129 are involved in binding the FMN isoalloxazine ring. Residue Trp105 is involved in FMN stabilization and destabilization Enterococcus faecalis
1.7.1.17 NADH
-
 Enterococcus faecalis