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Literature summary extracted from

  • Rohles, C.; Giesselmann, G.; Kohlstedt, M.; Wittmann, C.; Becker, J.
    Systems metabolic engineering of Corynebacterium glutamicum for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate (2016), Microb. Cell Fact., 15, 154 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.12.2 gene davB, recombinant expression in Corynebacterium glutamicum strain LYS-12, coexpression with Pseudomonas putida gene davA, encoding 5-aminovaleramidase Pseudomonas putida
3.5.1.30 gene davA, recombinant expression in Corynebacterium glutamicum, coexpression with gene davB from Pseudomonas putida encoding lysine 2-monooxygenase (EC 1.13.12.2) Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.13.12.2 additional information installation of an expression system for production of 5-aminovalerate in Corynebacterium glutamicum strain LYS-12 by coexpressing gene davA, encoding 5-aminovaleramidase, and davB, encoding lysine monooxygenase, resulting in strains AVA-1-3. 5-Aminovalerate production is established. Related to the presence of endogenous genes coding for 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase, 5-aminovalerate is partially converted to glutarate. Residual L-lysine is secreted as by-product. Putative gabT gene is deleted to enhance 5-aminovalerate production, method optimization and evaluation, overview Pseudomonas putida
3.5.1.30 additional information systems metabolic engineering of Corynebacterium glutamicum for construction of a recombinant microbial cell factory for the production of the carbon-5 platform chemicals 5-aminovalerate and glutarate, method development on basis of the lysine-hyperproducing strain Corynebacterium glutamicum LYS-12 and optimization, detailed overview. Related to the presence of endogenous genes coding for 5-aminovalerate transaminase (gabT) and glutarate semialdehyde dehydrogenase, 5-aminovalerate is partially converted to glutarate. Moreover, residual l-lysine is secreted as by-product. The issue of by-product formation is then addressed by deletion of the lysE gene, encoding the l-lysine exporter. Additionally, a putative gabT gene is deleted to enhance 5-aminovalerate production. Fed-batch fermentation. Method optimization, overview. Production of 28 g/l of 5-aminovalerate with a maximal space to time yield of 0.9 g/l per h from engineered AVA-3 strain Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.12.2 L-lysine + O2 Pseudomonas putida
-
 5-aminopentanamide + CO2 + H2O
-
 ?
3.5.1.30 5-aminopentanamide + H2O Pseudomonas putida
-
 5-aminopentanoate + NH3
-
 ?
3.5.1.30 5-aminopentanamide + H2O Pseudomonas putida ATCC 12633
-
 5-aminopentanoate + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.12.2 Pseudomonas putida
-
-
-
3.5.1.30 Pseudomonas putida B3IVI7
-
-
3.5.1.30 Pseudomonas putida ATCC 12633 B3IVI7
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.12.2 L-lysine + O2
-
 Pseudomonas putida 5-aminopentanamide + CO2 + H2O
-
 ?
3.5.1.30 5-aminopentanamide + H2O
-
 Pseudomonas putida 5-aminopentanoate + NH3
-
 ?
3.5.1.30 5-aminopentanamide + H2O
-
 Pseudomonas putida ATCC 12633 5-aminopentanoate + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.12.2 davB
-
 Pseudomonas putida
1.13.12.2 lysine monooxygenase
-
 Pseudomonas putida
3.5.1.30 5-aminovaleramidase
-
 Pseudomonas putida
3.5.1.30 5-aminovaleramide amidase
-
 Pseudomonas putida
3.5.1.30 DavA
-
 Pseudomonas putida

General Information

EC Number General Information Comment Organism
3.5.1.30 metabolism metabolic pathway design for the recombinant production of 5-aminovalerate and glutarate in Corynebacterium glutamicum starting from the pathway precursor L-lysine and involving 5-aminovaleramide amidase, overview Pseudomonas putida